Effects of orally applied butyrate bolus on histone acetylation and cytochrome P450 enzyme activity in the liver of chicken – a randomized controlled trial

Table 2 Effects of oral application of butyrate on kinetic properties of cytochrome P450 (CYP) enzymes in chicken ¹

Enzyme action	Responsible CYP	Kinetic parameter	Control	Butyrate 0.25 g/kg BW²	Butyrate 1.25 g/kg BW²	PB³
Aminopyrine N-demethylation	CYP2H/3A37	V_max (μmol/mg/min)	0.22 ± 0.06	0.21 ± 0.04	0.17 ± 0.03	0.38 ± 0.01^**
		K_M (mM)	1.07 ± 0.24	1.20 ± 0.33	0.57 ± 0.15	1.02 ± 0.14
Aniline-hydroxylation	CYP2H	V_max (μmol/mg/min)	0.11 ± 0.02	0.15 ± 0.04	0.13 ± 0.03	0.19 ± 0.01
		K_M (mM)	5.27 ± 0.48	12.25 ± 4.36	7.86 ± 2.55	3.27 ± 0.45

¹Values are shown as means ± SEM (control: n=9; butyrate, lower dose: n=10; butyrate, higher dose: n=9; PB: n=6).
²Butyrate = chickens treated with oral application of butyrate bolus in a lower (0.25 g/kg BW) and a higher (1.25 g/kg BW) dose.
³PB = chickens received intracoelomal phenobarbital injection (80 mg/kg BW) on days 20–24.
^**Significant difference, P<0.01.

ISSN: 1743-7075