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Table 2 Effects of oral application of butyrate on kinetic properties of cytochrome P450 (CYP) enzymes in chicken 1

From: Effects of orally applied butyrate bolus on histone acetylation and cytochrome P450 enzyme activity in the liver of chicken – a randomized controlled trial

Enzyme action Responsible CYP Kinetic parameter Control Butyrate 0.25 g/kg BW2 Butyrate 1.25 g/kg BW2 PB3
Aminopyrine N-demethylation CYP2H/3A37 Vmax (μmol/mg/min) 0.22 ± 0.06 0.21 ± 0.04 0.17 ± 0.03 0.38 ± 0.01**
   KM (mM) 1.07 ± 0.24 1.20 ± 0.33 0.57 ± 0.15 1.02 ± 0.14
Aniline-hydroxylation CYP2H Vmax (μmol/mg/min) 0.11 ± 0.02 0.15 ± 0.04 0.13 ± 0.03 0.19 ± 0.01
   KM (mM) 5.27 ± 0.48 12.25 ± 4.36 7.86 ± 2.55 3.27 ± 0.45
  1. 1Values are shown as means ± SEM (control: n=9; butyrate, lower dose: n=10; butyrate, higher dose: n=9; PB: n=6).
  2. 2Butyrate = chickens treated with oral application of butyrate bolus in a lower (0.25 g/kg BW) and a higher (1.25 g/kg BW) dose.
  3. 3PB = chickens received intracoelomal phenobarbital injection (80 mg/kg BW) on days 20–24.
  4. **Significant difference, P<0.01.