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Table 1 Characteristics and performance data of the primers used for reference gene-stability measure M and qPCR

From: Supplementation of carnitine leads to an activation of the IGF-1/PI3K/Akt signalling pathway and down regulates the E3 ligase MuRF1 in skeletal muscle of rats

Gene

Forward primer (5′→3′)

Product size (bp)

NCBI GenBank

Slope

R21

Efficiency2

M

 

Reverse primer (5′→3′)

  

Liver

Muscle

Liver

Muscle

Liver

Muscle

Liver

Muscle

Reference genes

ATP5B

GCACCGTCAGAACTATTGCT

203

NM_134364.1

−0.31

−0.34

0.99

0.99

2.04

2.00

0.069

0.149

 

GAATTCAGGAGCCTCAGCAT

          

CANX

CCAGATGCAGATCTGAAGAC

175

NM_172008.2

−0.32

−0.32

0.99

0.99

2.10

1.98

0.065

0.082

 

CTGGGTCCTCAATTTCACGT

          

MDH1

CAGACAAAGAAGAGGTTGCC

206

NM_033235.1

−0.32

−0.32

0.99

0.99

2.07

2.10

0.054

0.111

 

CGTCAGGCAGTTTGTATTGG

          

RPL13

CTTAAATTGGCCACGCAGCT

198

NM_031101.1

−0.23

−0.30

0.88

0.99

1.70

2.01

0.072

0.105

 

CTTCTCAACGTCTTGCTCTG

          

TOP1

GAAGAACGCTATCCAGAAGG

137

NM_022615.1

−0.29

−0.30

0.99

0.99

1.95

2.20

0.066

0.088

 

GCTTTGGGACTCAGCTTCAT

          

YWHAZ

GACGGAAGGTGCTGAGAAA

198

NM_013011.3

−0.30

−0.30

0.98

0.99

2.02

2.08

0.057

0.082

 

GCAGCAACCTCAGCCAAGT

          

Target genes

IGF-1

CCCGGGACGTACCAAAATGAGCG

354

NM_001082477.2

−0.29

 

0.99

 

1.97

   
 

ATGTCAGTGTGGCGCTGGGC

          

FBXO32

GACTGGACTTCTCGACTGCC

242

NM_133521.1

 

−0.29

 

0.98

 

1.94

  
 

GACTTGCCGACTCTCTGGAC

          

TRIM63

AAGGCAGCCACCCGATGTGC

110

NM_080903.1

 

−0.31

 

0.99

 

2.02

  
 

GCCTGGTGAGCCCCGAACAC

          
  1. 1Coefficient of determination of the standard curve.
  2. 2The efficiency was determined by [10-slope].
  3. ATP5B: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; CANX: calnexin; MDH1: soluble malate dehydrogenase; RPL13: ribosomal protein L13; TOP1: topoisomerase (DNA) I; YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; IGF-1: insulin-like growth factor 1; FBXO32: F-box protein 32; TRIM63: tripartite motif containing 63.
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Nutrition & Metabolism

ISSN: 1743-7075

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