|
Gene
|
Forward primer (5′→3′)
|
Product size (bp)
|
NCBI GenBank
|
Slope
|
R21
|
Efficiency2
|
M
|
|---|
| |
Reverse primer (5′→3′)
| | |
Liver
|
Muscle
|
Liver
|
Muscle
|
Liver
|
Muscle
|
Liver
|
Muscle
|
|---|
|
Reference genes
|
|
ATP5B
|
GCACCGTCAGAACTATTGCT
|
203
|
NM_134364.1
|
−0.31
|
−0.34
|
0.99
|
0.99
|
2.04
|
2.00
|
0.069
|
0.149
|
| |
GAATTCAGGAGCCTCAGCAT
| | | | | | | | | | |
|
CANX
|
CCAGATGCAGATCTGAAGAC
|
175
|
NM_172008.2
|
−0.32
|
−0.32
|
0.99
|
0.99
|
2.10
|
1.98
|
0.065
|
0.082
|
| |
CTGGGTCCTCAATTTCACGT
| | | | | | | | | | |
|
MDH1
|
CAGACAAAGAAGAGGTTGCC
|
206
|
NM_033235.1
|
−0.32
|
−0.32
|
0.99
|
0.99
|
2.07
|
2.10
|
0.054
|
0.111
|
| |
CGTCAGGCAGTTTGTATTGG
| | | | | | | | | | |
|
RPL13
|
CTTAAATTGGCCACGCAGCT
|
198
|
NM_031101.1
|
−0.23
|
−0.30
|
0.88
|
0.99
|
1.70
|
2.01
|
0.072
|
0.105
|
| |
CTTCTCAACGTCTTGCTCTG
| | | | | | | | | | |
|
TOP1
|
GAAGAACGCTATCCAGAAGG
|
137
|
NM_022615.1
|
−0.29
|
−0.30
|
0.99
|
0.99
|
1.95
|
2.20
|
0.066
|
0.088
|
| |
GCTTTGGGACTCAGCTTCAT
| | | | | | | | | | |
|
YWHAZ
|
GACGGAAGGTGCTGAGAAA
|
198
|
NM_013011.3
|
−0.30
|
−0.30
|
0.98
|
0.99
|
2.02
|
2.08
|
0.057
|
0.082
|
| |
GCAGCAACCTCAGCCAAGT
| | | | | | | | | | |
|
Target genes
|
|
IGF-1
|
CCCGGGACGTACCAAAATGAGCG
|
354
|
NM_001082477.2
|
−0.29
| |
0.99
| |
1.97
| | | |
| |
ATGTCAGTGTGGCGCTGGGC
| | | | | | | | | | |
|
FBXO32
|
GACTGGACTTCTCGACTGCC
|
242
|
NM_133521.1
| |
−0.29
| |
0.98
| |
1.94
| | |
| |
GACTTGCCGACTCTCTGGAC
| | | | | | | | | | |
|
TRIM63
|
AAGGCAGCCACCCGATGTGC
|
110
|
NM_080903.1
| |
−0.31
| |
0.99
| |
2.02
| | |
| |
GCCTGGTGAGCCCCGAACAC
| | | | | | | | | | |
- 1Coefficient of determination of the standard curve.
- 2The efficiency was determined by [10-slope].
- ATP5B: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; CANX: calnexin; MDH1: soluble malate dehydrogenase; RPL13: ribosomal protein L13; TOP1: topoisomerase (DNA) I; YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; IGF-1: insulin-like growth factor 1; FBXO32: F-box protein 32; TRIM63: tripartite motif containing 63.
