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Table 2 Adipose tissue gene expression levels in CB 1 +/+ and CB 1 -/- mice fed chow, a HF or a HF/FO diet during 6 weeks

From: Resistance to diet-induced adiposity in cannabinoid receptor-1 deficient mice is not due to impaired adipocyte function

  chow HF HF/FO
  CB 1 +/+ CB 1 -/- CB 1 +/+ CB 1 -/- CB 1 +/+ CB 1 -/-
Endocannabinoid system      
CB1 (Cnr1) 1.0 ± 0.1 ND 1.0 ± 0.1 ND 1.1 ± 0.2 ND
Napepld 1.0 ± 0.1 0.9 ± 0.1 1.4 ± 0.1# 1.3 ± 0.2# 1.2 ± 0.1 1.1 ± 0.1
Faah 1.0 ± 0.3 1.8 ± 0.5 1.1 ± 0.6 1.1 ± 0.4 1.0 ± 0.5 1.3 ± 0.6
Adipocyte proliferation and differentiation     
Pparγ2 (Pparg) 1.0 ± 0.2 1.0 ± 0.1 1.4 ± 0.1 1.2 ± 0.3 1.0 ± 0.1$ 0.9 ± 0.1
C/ebpα (Cebpa) 1.0 ± 0.1 1.0 ± 0.1 1.2 ± 0.0 1.3 ± 0.3 1.0 ± 0.1 0.9 ± 0.1
Ap2 (Fabp4) 1.0 ± 0.1 0.9 ± 0.1 1.5 ± 0.0# 1.2 ± 0.2 1.4 ± 0.1 1.2 ± 0.1
Adiponectin (Adipoq) 1.0 ± 0.2 0.9 ± 0.2 1.3 ± 0.1 1.3 ± 0.4 1.3 ± 0.1 1.0 ± 0.1
Trigyceride synthesis       
Srebp-1c (Srebf1) 1.0 ± 0.1 1.2 ± 0.1 1.5 ± 0.1# 1.8 ± 0.4 1.1 ± 0.0$ 1.1 ± 0.0
Acc1 (Acaca) 1.0 ± 0.2 1.1 ± 0.3 0.9 ± 0.1 0.9 ± 0.3 0.6 ± 0.1$ 0.5 ± 0.1#
Fas (Fasn) 1.0 ± 0.2 1.1 ± 0.2 1.1 ± 0.1 1.0 ± 0.3 0.7 ± 0.1$ 0.4 ± 0.0#
Scd1 1.0 ± 0.1 0.9 ± 0.1 2.8 ± 0.1# 3.1 ± 0.7# 0.9 ± 0.1$ 1.1 ± 0.1$
Pepck (Pck1) 1.0 ± 0.2 0.9 ± 0.1 1.0 ± 0.1 1.3 ± 0.4 1.1 ± 0.1 0.9 ± 0.1
Fatty acid uptake and transport     
Cd36 1.0 ± 0.1 1.1 ± 0.1 1.1 ± 0.0 1.2 ± 0.1 1.4 ± 0.2 1.1 ± 0.1
Fatp4 (Slc27a4) 1.0 ± 0.1 1.0 ± 0.1 1.2 ± 0.0 1.4 ± 0.2 1.2 ± 0.1 1.1 ± 0.1
Fatty acid hydrolysis     
Lpl 1.0 ± 0.3 0.9 ± 0.2 1.2 ± 0.1 0.9 ± 0.2 1.4 ± 0.2 1.0 ± 0.1
Gpihbp1 1.0 ± 0.1 1.2 ± 0.1 1.2 ± 0.1 1.2 ± 0.1 1.3 ± 0.1 0.9 ± 0.0
Angptl3 1.0 ± 0.1 0.9 ± 0.1 1.2 ± 0.1 1.1 ± 0.1 1.0 ± 0.1 1.0 ± 0.0
Angptl4 1.0 ± 0.1 1.0 ± 0.1 1.3 ± 0.1 1.2 ± 0.1 1.5 ± 0.1 1.1 ± 0.1
Apoc1 1.0 ± 0.2 1.2 ± 0.1 1.1 ± 0.0 1.1 ± 0.0 0.7 ± 0.1$ 0.9 ± 0.1
Apoc3 1.0 ± 0.2 0.7 ± 0.1 0.6 ± 0.1 0.4 ± 0.0 0.2 ± 0.0$ 0.2 ± 0.0#
Macrophage infiltration       
Cd68 1.0 ± 0.1 1.0 ± 0.1 1.2 ± 0.0 1.0 ± 0.1 1.3 ± 0.1 1.0 ± 0.1
Adipocyte lipolysis       
Hsl (Lipe) 1.0 ± 0.2 1.1 ± 0.2 1.2 ± 0.1 1.0 ± 0.2 1.2 ± 0.1 1.1 ± 0.1
Atgl (Pnpla2) 1.0 ± 0.2 1.1 ± 0.2 1.5 ± 0.2 1.4 ± 0.4 1.2 ± 0.1 1.2 ± 0.2
Fatty acid oxidation       
Cpt1a 1.0 ± 0.1 1.2 ± 0.2 1.6 ± 0.2# 1.2 ± 0.2 1.3 ± 0.1# 1.1 ± 0.1
  1. Expression levels were normalized to cyclophilin expression and values are given as means ± SEM for n = 4-8; # p < 0.05 compared to chow group of the same genotype, $ p < 0.05 compared to HF group of the same genotype (Student t-test). ND, non-detectable.
  2. General linear model analysis revealed overall effects for the following parameters (p < 0.05):
  3. Chow versus HF: aP2, Scd1, Srebp-1c.
  4. Chow versus HF/FO: Fas.
  5. HF versus HF/FO: Fas, Scd1, Srebp-1c.