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Table 2 Adipose tissue gene expression levels in CB 1 +/+ and CB 1 -/- mice fed chow, a HF or a HF/FO diet during 6 weeks

From: Resistance to diet-induced adiposity in cannabinoid receptor-1 deficient mice is not due to impaired adipocyte function

 

chow

HF

HF/FO

 

CB 1 +/+

CB 1 -/-

CB 1 +/+

CB 1 -/-

CB 1 +/+

CB 1 -/-

Endocannabinoid system

     

CB1 (Cnr1)

1.0 ± 0.1

ND

1.0 ± 0.1

ND

1.1 ± 0.2

ND

Napepld

1.0 ± 0.1

0.9 ± 0.1

1.4 ± 0.1#

1.3 ± 0.2#

1.2 ± 0.1

1.1 ± 0.1

Faah

1.0 ± 0.3

1.8 ± 0.5

1.1 ± 0.6

1.1 ± 0.4

1.0 ± 0.5

1.3 ± 0.6

Adipocyte proliferation and differentiation

    

Pparγ2 (Pparg)

1.0 ± 0.2

1.0 ± 0.1

1.4 ± 0.1

1.2 ± 0.3

1.0 ± 0.1$

0.9 ± 0.1

C/ebpα (Cebpa)

1.0 ± 0.1

1.0 ± 0.1

1.2 ± 0.0

1.3 ± 0.3

1.0 ± 0.1

0.9 ± 0.1

Ap2 (Fabp4)

1.0 ± 0.1

0.9 ± 0.1

1.5 ± 0.0#

1.2 ± 0.2

1.4 ± 0.1

1.2 ± 0.1

Adiponectin (Adipoq)

1.0 ± 0.2

0.9 ± 0.2

1.3 ± 0.1

1.3 ± 0.4

1.3 ± 0.1

1.0 ± 0.1

Trigyceride synthesis

      

Srebp-1c (Srebf1)

1.0 ± 0.1

1.2 ± 0.1

1.5 ± 0.1#

1.8 ± 0.4

1.1 ± 0.0$

1.1 ± 0.0

Acc1 (Acaca)

1.0 ± 0.2

1.1 ± 0.3

0.9 ± 0.1

0.9 ± 0.3

0.6 ± 0.1$

0.5 ± 0.1#

Fas (Fasn)

1.0 ± 0.2

1.1 ± 0.2

1.1 ± 0.1

1.0 ± 0.3

0.7 ± 0.1$

0.4 ± 0.0#

Scd1

1.0 ± 0.1

0.9 ± 0.1

2.8 ± 0.1#

3.1 ± 0.7#

0.9 ± 0.1$

1.1 ± 0.1$

Pepck (Pck1)

1.0 ± 0.2

0.9 ± 0.1

1.0 ± 0.1

1.3 ± 0.4

1.1 ± 0.1

0.9 ± 0.1

Fatty acid uptake and transport

    

Cd36

1.0 ± 0.1

1.1 ± 0.1

1.1 ± 0.0

1.2 ± 0.1

1.4 ± 0.2

1.1 ± 0.1

Fatp4 (Slc27a4)

1.0 ± 0.1

1.0 ± 0.1

1.2 ± 0.0

1.4 ± 0.2

1.2 ± 0.1

1.1 ± 0.1

Fatty acid hydrolysis

    

Lpl

1.0 ± 0.3

0.9 ± 0.2

1.2 ± 0.1

0.9 ± 0.2

1.4 ± 0.2

1.0 ± 0.1

Gpihbp1

1.0 ± 0.1

1.2 ± 0.1

1.2 ± 0.1

1.2 ± 0.1

1.3 ± 0.1

0.9 ± 0.0

Angptl3

1.0 ± 0.1

0.9 ± 0.1

1.2 ± 0.1

1.1 ± 0.1

1.0 ± 0.1

1.0 ± 0.0

Angptl4

1.0 ± 0.1

1.0 ± 0.1

1.3 ± 0.1

1.2 ± 0.1

1.5 ± 0.1

1.1 ± 0.1

Apoc1

1.0 ± 0.2

1.2 ± 0.1

1.1 ± 0.0

1.1 ± 0.0

0.7 ± 0.1$

0.9 ± 0.1

Apoc3

1.0 ± 0.2

0.7 ± 0.1

0.6 ± 0.1

0.4 ± 0.0

0.2 ± 0.0$

0.2 ± 0.0#

Macrophage infiltration

      

Cd68

1.0 ± 0.1

1.0 ± 0.1

1.2 ± 0.0

1.0 ± 0.1

1.3 ± 0.1

1.0 ± 0.1

Adipocyte lipolysis

      

Hsl (Lipe)

1.0 ± 0.2

1.1 ± 0.2

1.2 ± 0.1

1.0 ± 0.2

1.2 ± 0.1

1.1 ± 0.1

Atgl (Pnpla2)

1.0 ± 0.2

1.1 ± 0.2

1.5 ± 0.2

1.4 ± 0.4

1.2 ± 0.1

1.2 ± 0.2

Fatty acid oxidation

      

Cpt1a

1.0 ± 0.1

1.2 ± 0.2

1.6 ± 0.2#

1.2 ± 0.2

1.3 ± 0.1#

1.1 ± 0.1

  1. Expression levels were normalized to cyclophilin expression and values are given as means ± SEM for n = 4-8; # p < 0.05 compared to chow group of the same genotype, $ p < 0.05 compared to HF group of the same genotype (Student t-test). ND, non-detectable.
  2. General linear model analysis revealed overall effects for the following parameters (p < 0.05):
  3. Chow versus HF: aP2, Scd1, Srebp-1c.
  4. Chow versus HF/FO: Fas.
  5. HF versus HF/FO: Fas, Scd1, Srebp-1c.
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Nutrition & Metabolism

ISSN: 1743-7075

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