Open Access

Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity

  • Waleska Cláudia Dornas1,
  • Wanderson Geraldo de Lima1, 2,
  • Rinaldo Cardoso dos Santos3,
  • Melina Oliveira de Souza1,
  • Maísa Silva1,
  • Mirla Fiuza Diniz2 and
  • Marcelo Eustáquio Silva1, 3Email author
Nutrition & Metabolism20129:63

https://doi.org/10.1186/1743-7075-9-63

Received: 29 March 2012

Accepted: 27 June 2012

Published: 27 June 2012

Abstract

Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P < 0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.

Keywords

Fructose-fed rats High-salt diet Paraoxonase Atherosclerosis

Findings

Diabetes is a long-term disorder affecting the inner walls of arteries and is characterized by endothelial dysfunction and oxidative modification of low density lipoprotein (LDL) which may induce atherosclerosis [1]. In contrast, it is supposed that LDL particles can be protected from free radical-induced oxidation by an HDL linked enzyme, paraoxonase 1 (PON1).

Although the precise mechanism of PON1 effect is not yet completely known, it may possess anti-atherogenic and anti-inflammatory properties, resulting from its ability to destroy modified phospholipids and to prevent accumulation of oxidized lipids in lipoproteins [25]. In diabetes experimental models, it has been reported that PON1 enzymatic activity is decreased [6, 7] and reduced PON1 activity was suggested to play a role in the severity of coronary atherosclerosis [2]. Nonetheless, oxidative stress pathways have also been proposed to act in this process, with increased reactive oxygen species (ROS) production and/or impaired antioxidant defense, which may in turn lead to excessive peroxidation of polyunsaturated fatty acids contained in LDL particles.

Our laboratory has recently reported that a hypercholesterolemic diet in rats cause a significant reduction of PON1 activity, associated with increased oxidative stress [8]. Once rats fed a high dose of fructose are considered a nutritional model for insulin resistance [911], the present study investigated the effects of a high-salt diet in fructose-fed insulin-resistant rats on PON1 and its influence in serum and oxidative parameters. In addition the influence of the diets on the inflammatory cells profile was also assessed.

Twenty-nine Fisher male rats, weighing about 300 g, obtained from the School of Nutrition of the Federal University of Ouro Preto, were housed in a temperature and humidity controlled room with a 12:12-h light/dark cycle (lights on at 06.00 AM). Animals were initially divided into two groups. The control group (CON) received the standard AIN93 diet [12] and the high fructose-fed group (HF) was given supplemented fructose as a 20% solution instead of pure water ad libitum to induce insulin resistance. After 10 weeks, HF animals were divided into 2 groups: those that continued to receive only 20% fructose in water and those switched to the high fructose regimen + 8% NaCl in the standard AIN93 diet (HFS). After 20 weeks fasting rats were anesthetized and sacrificed. The experiments were approved by the institutional Ethics Committee with protocol number 068/2011 and were performed in accordance with the principles of the Brazilian College of Animal Experimentation [13].

To determine the levels of serum components, blood samples were collected in test tubes and centrifuged. Serum total protein, albumin, total cholesterol, HDL-cholesterol and triglyceride were assayed with colorimetric or enzymatic methods using commercially available kits Labtest (Lagoa Santa, MG, Brazil) # 99, 19, 76, 13 and 87, respectively. The atherogenic index was calculated as follows: total cholesterol - HDL cholesterol/HDL cholesterol.

PON activity was measured by a spectrophotometric assay using phenyl acetate as substrate as described by Beltowski et al. [14]. The enzymatic activity was calculated assuming the molar extinction coefficient of phenylacetate to be 1310 L . mol-1. cm-1. The results were expressed in units per milliliter, where 1 U of arylesterase hydrolyzes 1 mmol of phenylacetate per minute. Basal lipid peroxidation status was measured in the liver by TBARS assay [15].

A drop of blood was used to prepare blood smears, which were stained with Panotic Fast which use essential components dyes of Romanowsky [16]. Counts of 100 leukocytes were performed and the results were expressed as subtypes identified in polymorphonuclear (PMN), lymphocyte or monocyte cells.

The normality of the sample distribution for each continuous parameter was tested with the Kolmogorov-Smirnov test. The significance of any differences in proportions or medians was tested with Kruskal-Wallis test, and in means analysis of variance (ANOVA) was used, followed by Dunns and Tukey test, respectively. Pearson’s correlation coefficients were used to test the correlation among variables. A p- value of less than 0.05 was considered statistically significant.

A high-salt diet in fructose-fed insulin-resistant rats significantly decreased serum levels of total protein, albumin, total cholesterol and HDL-cholesterol after 20 wk of treatment in relation the control group as demonstrated in Table 1. Fructose-fed rats showed significantly higher triglyceride concentrations in both HF and HFS groups (P < 0.05) as compared to control rats. The atherogenic index of each experimental group was also calculated and increased 66% in HFS rats as compared to controls. PON1 activity markedly fell to 68% in those animals, as compared to control and HF rats (P < 0.05 and P < 0.01, respectively) (Table 1).
Table 1

Serum total protein, albumin, triglyceride, total cholesterol, HDL-cholesterol, atherogenic index and PON1 activity of experimental rats after 20 weeks of treatment

 

CON

HF

HFS

Number of rats/group

9

10

10

Total protein (g/dL)

7,0 ± 2,0a

6,3 ± 1,8a

4,9 ± 1,5b

Albumin (g/dL)

2,8 ± 0,2a

2,6 ± 0,3a

2,1 ± 0,2b

Triglyceride (mg/dL)

126,1 ± 30,8b

183,5 ± 58,7a

197,1 ± 55,9a

Total cholesterol (mg/dL)

97,9 ± 20,9a

98,0 ± 13,3a

69,3 ± 9,0b

HDL-cholesterol (mg/dL)

66,4 ± 10,6a

65,8 ± 9,4a

43,6 ± 12,17b

Atherogenic index

0,39 ± 0,12b

0,47 ± 0,11b

0,65 ± 0,26a

PON1 (g/dL)

64,45 ± 13,36a

65,44 ± 17,29a

44,3 ± 14,43b

Values (mean ± SD) were obtained from groups CON (AIN diet and water for 20 weeks); HF (AIN diet + 20% fructose solution in water for 20 weeks) and HFS (8% NaCl in AIN93 diet + 20% fructose solution in water for 10 weeks after previous treatment for a period of 10 weeks with HF). Different superscript letters within the same row indicate significant difference at P < 0.05 by one-way ANOVA followed by Tukey’s test. High-density lipoprotein (HDL); paraoxonase1 activity (PON1).

Increased oxidative stress was noted in the liver obtained from HFS rats, since TBARS levels were raised by 1.2 times (P < 0.05) as compared to controls (Figure 1A). When data from all experimental groups were analyzed together, the effects of all treatments on serum PON1 activity were positively correlated with total protein (r = 0.7728, P < 0.001) (Figure 1B), albumin (r = 0.8142, P < 0.001) (Figure 1C), total cholesterol (r = 0.6228, P < 0.001) (Figure 1D) and HDL-cholesterol (r = 0.6145, P < 0.001) (Figure 1E). In addition, a negative correlation was found between serum PON1 activity and liver TBARS levels (r = − 0.6262, P < 0.01) (Figure 1F).
Figure 1

(A) Liver TBARS levels; scatter plots illustrating the association between (B) PON1 and total protein and (C) albumin and (D) Total cholesterol and (E) HDL-cholesterol and (F) TBARS in experimental groups. Control (dark filled circle); high-fructose (dark filled triangle) and high-fructose + salt (dark filled square) rats. High-density lipoprotein (HDL); paraoxonase1 activity (PON1).

Differential leukocyte counts in the total blood smear in insulin-resistant rats treated or not with the high-salt diet showed a significant decrease in the lymphocyte profile in relation to the control group, but the percentage of polymorphonuclear cells (PMNs) remained unchanged. Salt treatment caused a significant increase in the monocyte profile in HF and HFS rats as compared to the CON rats (Table 2).
Table 2

Polymorphonuclear cells, lymphocytes and monocytes count of experimental rats after 20 weeks of treatment

 

CON

HF

HFS

PMN cells (%)

5-15

3-27

7-23

Lymphocytes (%)

19-58

4-33**

7-20***

Monocytes (%)

27-76

36-85**

40-83*

Values were obtained from groups CON (AIN diet and water for 20 weeks); HF (AIN diet + 20% fructose solution in water for 20 weeks), and HFS (8% high salt in AIN93 diet + 20% fructose solution in water for 10 weeks after previous treatment for a period of 10 weeks with HF). Kruskall Wallis test followed by Dunns test. Values indicate lowest and highest percentage of cells counted respectively. * P < 0.05 ; ** P < 0.01 ; *** P < 0.001 . Polymorphonuclear (PMN) cells.

In this study we observed a decrease in serum PON1 activity in insulin-resistant rats on a high-salt diet and an association among PON1, oxidative stress and inflammation, which can occur during the development of atherosclerosis.

HDL is the most powerful independent negative predictor of cardiovascular events. The protective effects of HDL have been first attributed to its capacity to promote cellular cholesterol efflux from peripheral cells and deliver it to the liver for excretion, a process known as reverse cholesterol transport [1]. Furthermore HDL has also attracted particular attention because of its antioxidative potential. Interestingly this work sustains the relationship between low paraoxonase activity and diseases with low antioxidant defense and excessive lipid peroxidation since paraoxonase may act protectively in atherogenesis by hydrolyzing some products of lipid peroxidation and consequently by limiting LDL oxidation and foam cell formation [17].

In our model, serum albumin and protein levels are reduced probably due to protein synthesis disruption in the liver as observed by Shinn and Moon [18]. The damaged hepatocytes are potent sources of reactive oxygen intermediates and oxidative stress is important in many types of liver injury. Lipid peroxidation, which was found increased in this study, could change the properties of biological membranes, resulting in severe cell damage and play a significant role in the mechanisms related to the alterations observed by us, as it has been previously documented [19].

In rats, unlike in humans and rabbits, plasma triglycerides and cholesterol are transported predominantly by HDL [14] and fructose per se, after having reached the liver, may contribute to the increase in serum triglyceride as demonstrated in this study and by others authors [9, 20, 21]. We showed that low PON1 activity is associated with decreased HDL levels and this contributes to the hypothesis that PON1 is involved in the preservation of HDL. Since PON1 exerts a protective effect against oxidative stress, it is logical to find an association between this enzyme and liver impairment. PON1 activity decreased while lipid peroxidation increased in HFS rats, suggesting that PON1 activity may be involved in the defense against free radical production in liver organelles. Moreover, the atherogenic index of these animals indicated them to be more susceptible to the development of atherosclerosis. However the results of this study showed that PON1 activity towards synthetic substrates reflects its relationship to oxidative stress by means of the marker used but this hypothesis needs support by means of further investigation.

A previous study has shown that PON1 protects against atherosclerosis, by its ability to reduce macrophage foam cell formation, via reducing oxidative stress and stimulation of cholesterol efflux from macrophages [22]. The present study proposes that oxidatively modified LDL impair endothelial function, stimulate inflammatory response of monocytes/macrophages, activate migration and proliferation of vascular smooth muscle cells and induce immune response, as indicated by the increase of monocytes in HF and HFS groups. Therefore, inactivation of PON1 in HFS rats could reduce the ability of HDL to inhibit LDL modification and also decrease the ability of HDL to inhibit monocytic-endothelial interactions. Both conditions appear to be important in the inflammatory response in arterial wall cells, as demonstrated by the change in lymphocyte profile in relation to the control group, since this is a common finding during the systemic inflammatory response. In addition, clinical and animal studies suggest that low lymphocyte count plays a putative role in chronic inflammation [23].

Our dietetic model was chosen due its relevance to human nutrition because it mimics a common Western diet, with high consumption of sugar drinks and salty foods and this study is the first one to show an association between paraoxonase status and salt overload in insulin-resistant rats. This supports the relationship between low PON1 activity and diseases with low antioxidant defense and excessive lipid peroxidation. Paraoxonase may perform protectively in atherogenesis by hydrolyzing some products of lipid peroxidation and consequently by limiting LDL oxidation and foam cell formation [24]. This allows us to hypothesize that paraoxonase activity may lead to failure in the protection of LDL oxidation in insulin-resistant subjects on a high-salt diet. Thus the lack of prevention of LDL oxidation by HDL could be explained by the quantitative alteration of HDL, since PON1 generally seems to contribute to the antioxidant properties of HDL.

In conclusion, the present study indicates that a high-salt diet in fructose-fed rats leads to lowered serum PON1 activity, with deleterious effects on the antioxidant defense system. Furthermore, we suggest that a change in blood cells profile would reflect the oxidative stress detected. These findings we believe to be relevant in the field of human nutrition.

Abbreviations

HDL: 

High-density lipoprotein

LDL: 

Low-density lipoprotein

PMNs: 

Polymorphonuclear

PON: 

Paraoxonase1

ROS: 

Reactive oxygen species

TBARS: 

Thiobarbituric acid reactive substances.

Declarations

Authors’ Affiliations

(1)
Research in Biological Sciences - NUPEB, Federal University of Ouro Preto
(2)
Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto
(3)
Department of Foods, School of Nutrition, University of Ouro Preto

References

  1. Mertens A, Holvoet P: Oxidized LDL and HDL: antagonists in atherogenesis. FASEB J. 2001, 15: 2073-2084. 10.1096/fj.01-0273rev.View ArticleGoogle Scholar
  2. Durrington PN, Mackness B, Mackness MI: Paraoxonase and atherosclerosis. Arterioscler Thromb Vasc Biol. 2001, 21: 473-480. 10.1161/01.ATV.21.4.473.View ArticleGoogle Scholar
  3. Navab M, Ananthramaiah GM, Reddy ST, Van Lenten BJ, Ansell BJ, Fonarow GC, Vahabzadeh K, Hama S, Hough G, Kamranpour N, Berliner JA, Lusis AJ, Fogelman AM: The oxidation hypothesis of atherogenesis: the role of oxidized phospholipids and HDL. J Lipid Res. 2004, 45: 993-1007. 10.1194/jlr.R400001-JLR200.View ArticleGoogle Scholar
  4. Richter RJ, Jarvik GP, Furlong CE: Paraoxonase 1 status as a risk factor for disease or exposure. Adv Exp Med Biol. 2010, 660: 29-35. 10.1007/978-1-60761-350-3_4.View ArticleGoogle Scholar
  5. Précourt LP, Amre D, Denis MC, Lavoie JC, Delvin E, Seidman E, Levy E: The three-gene paraoxonase family: physiologic roles, actions and regulation. Atherosclerosis. 2011, 214: 20-36. 10.1016/j.atherosclerosis.2010.08.076.View ArticleGoogle Scholar
  6. Silva M, Lima WG, Silva ME, Pedrosa ML: Effect of streptozotocin on the glycemic and lipid profiles and oxidative stress in hamsters. Arq Bras Endocrinol Metabol. 2011, 55: 46-53. 10.1590/S0004-27302011000100006.View ArticleGoogle Scholar
  7. Alp H, Varol S, Celik MM, Altas M, Evliyaoglu O, Tokgoz O, Tanrıverdi MH, Uzar E: Protective effects of beta glucan and glicazide on brain tissue and sciatic nerve of diabetic rats induced by streptozonin. Exp Diabetes Res. 2012, 2012: 230342-View ArticleGoogle Scholar
  8. de Souza MO, Silva M, Silva ME, Oliveira Rde P, Pedrosa ML: Diet supplementation with acai (Euterpe oleracea Mart.) pulp improves biomarkers of oxidative stress and the serum lipid profile in rats. Nutrition. 2010, 26: 804-810. 10.1016/j.nut.2009.09.007.View ArticleGoogle Scholar
  9. Thorburn AW, Storlien LH, Jenkins AB, Khouri S, Kraegen EW: Fructose-induced in vivo insulin resistance and elevated plasma triglyceride levels in rats. Am J Clin Nutr. 1989, 49: 1155-1163.Google Scholar
  10. Mayes PA: Intermediary metabolism of fructose. Am J Clin Nutr. 1993, 58: 754S-765S.Google Scholar
  11. Bezerra RM, Ueno M, Silva MS, Tavares DQ, Carvalho CR, Saad MJ: A high fructose diet affects the early steps of insulin action in muscle and liver of rats. J Nutr. 2000, 130: 1531-1535.Google Scholar
  12. Reeves PG, Nielsen FH, Fahey GC: AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition and hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993, 123: 1939-1951.Google Scholar
  13. Colégio Brasileiro de Experimentação Animal: Princípios éticos na experimentação animal do Colégio Brasileiro de Experimentação Animal. 1991, COBEA, São PauloGoogle Scholar
  14. Bełtowski J, Wójcicka G, Mydlarczyk M, Jamroz A: Cerivastatin modulates plasma paraoxonase/arilesterase activity and oxidant-antioxidant balance in the rat. Pol J Pharmacol. 2002, 54: 143-150.Google Scholar
  15. Buege JA, Aust SD: Microsomal lipid peroxidation. Methods Enzymol. 1978, 52: 302-310.View ArticleGoogle Scholar
  16. de Oliveira RB, de Paula DA, Rocha BA, Franco JJ, Gobbo-Neto L, Uyemura SA, dos Santos WF, Da Costa FB: Renal toxicity caused by oral use of medicinal plants: the yacon example. J Ethnopharmacol. 2011, 27: 434-441.View ArticleGoogle Scholar
  17. Watson A, Berliner JA, Hama SY, La Du BN, Fuall KF, Fogelman AM, Navab M: Protective effect of high density lipoprotein associated paraoxonase: inhibition of the biological activity of minimally oxidized low density lipoprotein. J Clin Invest. 1995, 96: 2882-2891. 10.1172/JCI118359.View ArticleGoogle Scholar
  18. Shin MO, Moon JO: Effect of dietary supplementation of grape skin and seeds on liver fibrosis induced by dimethylnitrosamine in rats. Nutr Res Pract. 2010, 4: 369-374. 10.4162/nrp.2010.4.5.369.View ArticleGoogle Scholar
  19. Camps J, Marsillach J, Joven J: Measurement of serum paraoxonase-1 activity in the evaluation of liver function. World J Gastroenterol. 2009, 15: 1929-1933. 10.3748/wjg.15.1929.View ArticleGoogle Scholar
  20. Busserolles J, Gueux E, Rock E, Demigné C, Mazur A, Rayssiguier Y: Oligofructose protects against the hypertriglyceridemic and pro-oxidative effects of a high fructose diet in rats. J Nutr. 2003, 133: 1903-1908.Google Scholar
  21. D’Angelo G, Elmarakby AA, Pollock DM, Stepp DW: Fructose feeding increases insulin resistance but not blood pressure in Sprague–Dawley rats. Hypertension. 2005, 46: 806-811. 10.1161/01.HYP.0000182697.39687.34.View ArticleGoogle Scholar
  22. Rosenblat M, Karry R, Aviram M: Paraoxonase 1 (PON1) is a more potent antioxidant and stimulant of macrophage cholesterol efflux when present in HDL than in lipoprotein-deficient serum: relevance to diabetes. Atherosclerosis. 2006, 187: 74-81.Google Scholar
  23. Núñez J, Miñana G, Bodí V, Núñez E, Sanchis J, Husser O, Llàcer A: Low lymphocyte count and cardiovascular diseases. Curr Med Chem. 2011, 18: 3226-3233. 10.2174/092986711796391633.View ArticleGoogle Scholar
  24. Bełtowski J, Wójcicka G, Mydlarczyk M, Jamroz A: The effect of peroxisome proliferator-activated receptors alpha (PPARalpha) agonist, fenofibrate, on lipid peroxidation, total antioxidant capacity, and plasma paraoxonase 1 (PON1) activity. J Physiol Pharmacol. 2002, 53: 463-475.Google Scholar

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© Dornas et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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