Fig. 3

H&E staining to observe the trabecular number and number of adipocytes from rat tibial sections. (a) Representative images are shown. Scale bar, 100 μm. The black arrows indicate the trabecular bone structure. The red arrows represent adipocytes. (b) The trabecular number (N. trabecular) per square millimetre was evaluated (n = 5). (c) The number of adipocytes (N. adipocytes) per square millimetre was evaluated (n = 5). **P < 0.01, *P < 0.05 versus the NC group and ## P < 0.01, # p < 0.05 versus the HFD group by one-way ANOVA