Flaxseed oil ameliorated high-fat-diet-induced bone loss in rats by promoting osteoblastic function in rat primary osteoblasts

Table 2 Primer sequences used for the determination of gene expression

Gene (Rats)	Primer sequence((5′ - 3′)	Product bp
β-catenin	Forward CATCACCACGCTGCATAATC	156
β-catenin	Reverse GAGCTTGCTTTCCTGATTGC
RUNX2	Forward CATGGCCGGGAATGATGAG	148
RUNX2	Reverse TGTGAAGACCGTTATGGTCAAAGTG
os0074erix	Forward CACCCATTGCCAGTAATCTTCGT	97
os0074erix	Reverse GGACTGGAGCCATAGTGAGCTTCT
β-actin	Forward ACCCAGATCATGTTTGAGAC	99
β-actin	Reverse GTCAGGATCTTCATGAGGTAGT

β-catenin, catenin beta1; RUNX2, runt-related transcription factor 2; osterix, Sp7 transcription factor; β-actin, actin, beta

ISSN: 1743-7075