Fig. 2

BCAA administration reduced postoperative hyperglycemia and improved postoperative insulin resistance. A Blood glucose, B plasma insulin and C plasma glucagon are shown(n = 8). D HOMA‐IR, E HOMA-β and F HOMA-ISI indices were calculated from blood glucose and plasma insulin level (n = 8). The protein levels of p-AKT308, p-AKT473, AKT, p-P70S6K in G liver, H muscle and I adipose tissue were analyzed by Western Blot and quantified using ImageJ software (J, K, L), β-actin was used as an endogenous control (n = 6). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001, ns, not significant. BCAA: branched-chain amino acids, CON: control, AKT: Protein kinase B, P70S6K: P70 ribosomal protein S6 kinase