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A metabolic link between mitochondrial ATP synthesis and liver glycogen metabolism: NMR study in rats re-fed with butyrate and/or glucose



Butyrate, end-product of intestinal fermentation, is known to impair oxidative phosphorylation in rat liver and could disturb glycogen synthesis depending on the ATP supplied by mitochondrial oxidative phosphorylation and cytosolic glycolysis.


In 48 hr-fasting rats, hepatic changes of glycogen and total ATP contents and unidirectional flux of mitochondrial ATP synthesis were evaluated by ex vivo31P NMR immediately after perfusion and isolation of liver, from 0 to 10 hours after force-feeding with (butyrate 1.90 mg + glucose 14.0 mg.g-1 body weight) or isocaloric glucose (18.2 mg.g-1 bw); measurements reflected in vivo situation at each time of liver excision. The contribution of energetic metabolism to glycogen metabolism was estimated.


A net linear flux of glycogen synthesis (~11.10 ± 0.60 μmol glucosyl units.h-1.g-1 liver wet weight) occurred until the 6th hr post-feeding in both groups, whereas butyrate delayed it until the 8th hr. A linear correlation between total ATP and glycogen contents was obtained (r2 = 0.99) only during net glycogen synthesis. Mitochondrial ATP turnover, calculated after specific inhibition of glycolysis, was stable (~0.70 ± 0.25 μmol.min-1.g-1 liver ww) during the first two hr whatever the force-feeding, and increased transiently about two-fold at the 3rd hr in glucose. Butyrate delayed the transient increase (1.80 ± 0.33 μmol.min-1.g-1 liver ww) to the 6th hr post-feeding. Net glycogenolysis always appeared after the 8th hr, whereas flux of mitochondrial ATP synthesis returned to near basal level (0.91 ± 0.19 μmol.min-1.g-1 liver ww).


In liver from 48 hr-starved rats, the energy need for net glycogen synthesis from exogenous glucose corresponds to ~50% of basal mitochondrial ATP turnover. The evidence of a late and transient increase in mitochondrial ATP turnover reflects an energetic need, probably linked to a glycogen cycling. Butyrate, known to reduce oxidative phosphorylation yield and to induce a glucose-sparing effect, delayed the transient increase in mitochondrial ATP turnover and hence energy contribution to glycogen metabolism.


Among the short chain fatty acids (SCFA), butyrate is a natural nutrient found in food (i.e. butter and milk product) and is also produced physiologically (from 40 to 100 mmol) from intestinal fermentation of fiber [1]. Recent findings in the field of gut microbial flora strongly suggest that the symbiotic relationship between the intestinal microbiota and the human host can influence health [2]. Gut microbiota clearly affect the host nutritional metabolism with consequences for energy storage, which implies mechanistic interactions between events occurring in the colon and the regulation of energy metabolism [3].

The main site for butyrate metabolism is the liver since hepatic removal close to 100% has been evidenced in Wistar rats adapted to a high-fiber diet [4]. A recent in vivo study in human [5] evidenced that SCFAs were released by the gut (34.9 ± 9.1 μmol. kg-1 body weight. h-1) in the circulatory system, while the gut butyrate release was counterbalanced by butyrate hepatic uptake (-3.8 ± 1.6 μmol. kg-1 body weight. h-1). This indicated that the liver is highly involved in butyrate metabolism.

Fatty acids (FA) are both substrates and effectors of the hepatic oxidative pathways. They are β-oxidized in mitochondria and some of them (namely long chain fatty acids) are known to have a decoupling-like effect on mitochondrial oxidative phosphorylation [6, 7]. We previously showed that the direct perfusion of isolated and perfused rat liver with short and medium chain FA (butyrate, octanoate) decreased the ATP content [8]. More recently, we demonstrated in isolated rat liver that a butyrate perfusion of the organ decreases the oxidative phosphorylation yield in the whole organ by decreasing the mitochondrial synthesis flux of ATP [9].

The liver is the main site of glycogen contribution to the regulation of blood glucose. Glycogen content varies during the day, generally increasing from a nadir at the end of the post-absorptive period to a maximal content about 4-5 hours after a meal. Glycogen synthesis, which is located in cytosol, depends on the UTP supply and hence on ATP supply resulting from both mitochondrial oxidative phosphorylation and cytosolic glycolysis. There is in isolated rat liver a linear, positive, glucose-dependent and insulin-dependent correlation between the change in ATP content and that of glycogen [10]. Glucose or glycogen cycling may also occur [11], raising the question of the contribution of ATP turnover owing to the large energy cost of the gluconeogenesis pathway. Studies have been performed with fatty acids concerning the hepatic fluxes of glucose autoregulation which could implicate a glucose or glycogen cycling [1216]; however, to our knowledge, nothing is reported about the energetic cost of the effect of fatty acid on a potential cycling. Owing to the impairing effect of butyrate on oxidative phosphorylation, it can be hypothesized that butyrate disturbs the energy supply necessary for glycogen synthesis.

The present study sought to evaluate the effects of butyrate added to glucose feeding in rat on mitochondrial ATP synthesis in the liver and to determine whether butyrate could interact with glycogen storage, the protocol reflecting in vivo situation. Indeed, the originality of the present work was to monitor the changes of mitochondrial ATP turnover within a long post-feeding period, the changes reflecting integrated physiological responses of the whole liver to the kind of diet. It differed from our previous estimation of ATP turnover performed ex vivo in which the substrates were added to the perfusate of the isolated liver, excluding all digestive processes and regulations at the whole-body scale [8, 9].

Recent nuclear magnetic resonance (NMR) developments made it possible to study hepatocellular rates of global ATP synthesis in vivo by using magnetization saturation transfer method [17] or on hepatic freeze-trapped biopsies subsequently treated for NMR analysis by cryo-NMR method [18]. Magnetization saturation transfer cannot be used to estimate the mitochondrial contribution to ATP supply [19] because the rate of liver oxidative phosphorylation is too slow to be accurately evaluated by this method [20]. Moreover, these methods do not allow discrimination between mitochondrial and glycolytic ATP production, and the chemical ischemia (specific inhibition of oxidative phosphorylation) coupled with NMR spectroscopy is the only method leading to such a discrimination [9, 21].

In the present study, we monitor in glycogen-depleted liver of 48 hr-fasted rats, the glycogen recovery and the changes in mitochondrial ATP turnover induced by feeding of 48 hr-fasted rats with glucose alone or glucose plus butyrate. Hence, glycogen content, total ATP content and mitochondrial ATP supply were evaluated on the perfused and isolated liver, immediately after sacrifice.

Methods and materials


Glucose and butyrate were purchased from Sigma Chemical (St. Louis, Missouri, USA) except where otherwise specified.


Male Wistar rats (Centre d'élevage Depré, St Doulchard, France) weighing 90-120 g were fed ad libitum with a balanced diet: carbohydrates (65%), proteins (16%), water (12%), minerals (5%), fibers (4%) and lipids (3%) amounting to 12.75 MJ/kg food as previously described [8] (Table 1). They were housed in hanging cages in a room with constant airflow, controlled temperature (21-23°C) and hygrometry, and a 12 hr light/dark system.

Table 1 Formulation of rat diet

Specific force-feeding

Rats were fasted for 48 hr in order to totally deplete their hepatic glycogen store; a fasting period of 48 hr is currently used in various protocols concerning liver metabolic studies in rats [2224]. They were then fed with an intragastric bolus of one of the following mixtures: (i) force-feeding consisting in 18.2 mg glucose/g body weight, or (ii) force-feeding consisting in 14 mg glucose +1.9 mg butyrate/g body weight calculated to be isocaloric to force-feeding with glucose alone (7.28 cal). A significant difference (-23%) was thus obtained in the energy supply of the glucidic component. All mixtures were diluted with water for a total force-feeding (FF) volume of 1.8 ml/100 g body weight, thus respecting the maximal recommendations of 20 ml/kg of body weight. Intragastric administration with a cannula (Harvard apparatus; 16 gauge diameter; 4 inches long) was performed within 1 minute. This mode of administration, which avoids the repulsion linked to the taste of butyrate, allows the consumption of dietary nutrients to be rigorously controlled [25].

The laboratory is licensed for animal experiments (French Agriculture Department). The protocol complied with 1999 UFAW guidelines [26] and was approved by the Regional Ethics Committee for Animal Experiments in our University.

Preparation of animals for NMR measurements

Animals were anaesthetized by intraperitoneal injection of pentobarbital sodium (50 mg/kg of rat) at different times from the onset to the 10th hr of feeding. To avoid ischemic degradation of ATP, liver was perfused through the portal vein and then excised for NMR measurements. The number of rats (n) varied from 6 to 12 for each time point. The protocol is summarized in Figure 1.

Figure 1
figure 1

Sequences of protocol applied to measure resynthesis of total ATP and glycogen contents, and unidirectional flux of mitochondrial ATP synthesis. All the processes post-feeding occurred in vivo in integrated physiological conditions. The ex vivo step is the only way to estimate the mitochondrial contribution to ATP synthesis. The ex vivo step performed rapidly at each time post force-feeding reflects the in vivo situation. The total ATP and glycogen contents in controls fed ad libitum were 2.70 ± 0.30 and 74.97 ± 11.17 μmol/g liver wet weight, respectively. In 48-hr fasted controls, total ATP and glycogen contents were 0.95 ± 0.29 and 0.80 ± 0.10 μmol/g liver ww, respectively. In the force-feeding step, the bolus (expressed per g body weight) was isocaloric (7.28 kcal): 18.2 mg glucose or 14 mg glucose + 1.9 mg butyrate.

The liver ex vivo antegrade perfusion technique through the portal vein was performed as essentially described by Exton [27] and then modified for NMR spectroscopy [2830]. Briefly the liver (4-6 g) was perfused with isotonic Krebs-Henseleit buffer containing glucose (30 mmol/L) and insulin (120 mUI/L) [12, 31] (glucose concentration was chosen to maintain a high level of energetic metabolism and to avoid that its concentration becomes limiting for the velocity of energetic metabolism); the perfusate was pumped through a thermostatically controlled membrane oxygenator gassed with 95% O2 - 5% CO2 (37°C), at a flow perfusion of 4 ml/min.g liver wet weight [32, 9, 10] in a non recirculation mode. In order to avoid interference with non-hepatic ATP and the above conditions ensuring good oxygenation, no erythrocytes were used. The perfused liver was then excised from the rat abdomen and transferred to a 20 mm-diameter NMR cell.

NMR methodology

Determinations of total ATP and glycogen contents and of mitochondrial ATP turnover at each time point after feeding have been performed immediately after liver excision, thereby reflecting in vivo situation at the time of liver excision.

The spectra were obtained using a 31P/13C double-tuned 20 mm probe operating at 9.4 T. Liver ATP content was monitored by 31P NMR and carbohydrate content in natural abundance was assessed by 13C NMR. 31P and 13C NMR spectra were recorded at 161.9 and 100.6 MHz respectively on a DPX400 spectrometer (Bruker). The magnetic field was adjusted to the water proton signal. 31P NMR spectra were obtained without proton decoupling (each spectrum: 148 free induction decays, FIDs, 1 min 20 sec) throughout the sequence protocol. During inhibitor addition sequences, spectra were acquired every 20 sec (40 FIDs) in order to increase the determination accuracy. Radiofrequency pulses (70° flip angle) and 10,000 Hz spectral width were used for data acquisition. 13C NMR spectra were proton-decoupled using a gated bi-level mode. 13C NMR spectra were obtained (200 FIDs, 3 min acquisition time) from a 66° radiofrequency pulse repeated every second (25,000 Hz spectral width). Lorentzian line broadening of 15 Hz was applied before Fourier transformation for both 31P and 13C NMR spectra. Assignments of chemical shifts and the metabolite quantitation method were described elsewhere [810, 30].

Only livers maintaining a total ATP/Pi ratio, measured by 31P NMR, during the first 10 minutes of perfusion were kept for entire protocol (total perfusion duration: about 30 min).

Determination of mitochondrial ATP synthesis

At each time, the whole liver total ATP content reflects a dynamic equilibrium between ATP synthesis and consumption. In this condition, suppression of cytosolic and mitochondrial ATP production makes it possible to calculate the in situ rate (R) of mitochondrial ATP turnover [21]. For this purpose, perfusion was performed by first using iodacetate (IAA, 0.5 mmol/L, 2 min) to inhibit the couple glyceraldehyde 3-phosphate dehydrogenase/phosphoglycerate kinase (glycolysis inhibition). After 5 min to reach a new steady state of ATP content (without glycolytic ATP contribution), KCN (2.5 mmol/L, 10 min) was added to inhibit cytochrome oxidase (oxidative phosphorylation) leading to a fall of ATP content.

Since some authors have suspected non specific effects of IAA [18], we have previously performed a work of validation demonstrating that used in above conditions of both concentration and delay, IAA had no significant effect on other pathway than glycolysis [9]; in particular, we had measured only a transient (5 min) VO2 increase (<10 ± 2%) which returned to baseline. We have also validated KCN use by kinetic studies of liver ATP content in presence of carboxyatractyloside [9] demonstrating that in response to the large KCN-induced decrease of proton motive force, liver cytosolic ATP was not hydrolyzed in situ by mitochondria.

Calculation of mitochondrial ATP turnover: after KCN addition, the mitochondrial ATP content fell according to the following equation: ATP = A.exp-kt, (A, expressed in μmol.g-1 liver wet weight, being the ATP content at the time of KCN addition). The mitochondrial ATP turnover was R(t0) = -A.k (with k expressed in min-1 and R expressed in μmol. min-1.g-1 liver wet weight) since ATP synthesis Rate = ATP consumption Rate at the onset of KCN addition (t0).


All results were expressed as means ± SEM. Statistical analysis was performed using one-way analysis of variance (ANOVA) for all data analysis. A post-hoc t-test was performed following the analysis of variance (P value lower than 0.05 was considered to be significant).


Change in liver glycogen content after feeding

After 48 hr starvation, the liver glycogen content was undetectable with NMR (Figure 2) compared to a liver isolated from a control rat fed ad libitum (Figure 3A). In fasting animals, the glycogen content determined by biochemical assay was 0.85 ± 0.34 μmol.g-1 liver ww [33]. From force-feeding, glycogen increased regularly in a linear time-dependent manner (11.10 ± 0.60 μmol.h-1.g-1 liver ww, R2 = 0.95) until the 6th hr post-feeding for the glucose group (Figure 2), and the 8th hr in the butyrate group. Glycogen NMR measurements were validated by comparing them with in vitro enzymatic measurements on the liver extracts for each time after force-feeding; glycogen content and time evolution were not different between the two methods [33].

Figure 2
figure 2

Kinetic of liver glycogen resynthesis according to type of force-feeding in fasted rats (expressed in glucosyl units). m ± SEM. n varied from 6 to 12 for each delay. Isocaloric (7.28 cal) force-feeding (expressed per g body weight) consisted of 18.2 mg glucose or 14 mg glucose + 1.9 mg butyrate. Experimental conditions reflect the in vivo situation at each time of liver excision. Labeled means at a time without a common letter differ (t-test), P = 0.05. *Different from previous time in the Glucose group and in the Butyrate group, P < 0.01. **Different from 6th hour in Glucose+Butyrate group, P = 0.035.

Figure 3
figure 3

Typical NMR spectra of perfused and isolated liver of rat fed ad libitum. 3A: 13C NMR spectrum. An external silicone reference gives a resonance at 0 ppm. Peak assignments: (a and h) fatty acids chains; (b) C-1 glycogen; (c) C-1α and C-1β glucose (mainly exogenous glucose of the perfusate); (d) glucose and glycogen (C-3β, C-5β glucose, glycogen; C-2 glucose; C-3α glucose; C-2, C-5α glucose, C-5 glycogen; C-4αβ glucose, glycogen); (e) C-6 glucose, glycogen; (f) choline; (g) ethanolamine. The chemical shift scale δ is given in parts per million (ppm) according to: chemical shift (Hz) = δ(ppm) × A(MHz), A being the frequency of the spectrometer. The unit ppm is used owing to the order value (10-6) of a constant characterizing the chemical nature of the nucleus. This scale allows easy comparison between spectra obtained in spectrometers operating at different magnetic fields. 3B. 31P NMR spectrum. Major resonances are assigned to (a) phosphomonoesters, (b) phosphocholine, (c) intracellular inorganic phosphate, (d) glycerol-3- phosphorylcholine and glycerol-3-phosphorylethanolamine, (e) nucleoside-'-triphosphates (γNTP) and diphosphates (βNDP), (f) α-NTP and β-NDP, (g, h) nicotinamide adenine dinucleotide and uridine-'-diphosphoglucose, (i) β-NTP.

A net glycogenolysis was observed from the 6th hr when animals were fed with glucose alone (-1.60 ± 1.00 μmol.h-1.g-1 liver ww, R2 = 0.76, n = 10) and from the 8th hr in the butyrate group (-19.80 ± 2.40 μmol.h-1.g-1 ww). At the 10th hr (n = 9) the glycogen content reached a level similar to that in isocaloric glucose force-feeding.

Correlation between total ATP and glycogen contents during the glycogen synthesis period

A typical 31P NMR spectrum of liver fed rat is shown in Figure 3B. NMR depicted mainly nuclei of the active biochemical forms of molecules. Liver total ATP content in ad libitum fed rats was 2.42 ± 0.32 μmol.g-1 liver ww in entire perfused organ, corresponding to 2.70 ± 0.30 μmol.g-1 liver ww when measured by 31P NMR on perchloric extracts from freeze-clamped perfused livers (n = 15), due to the demonstrated NMR-visibility of βATP near 90% [34]. After 48 hr starvation, the total ATP content measured in minutes following the force-feeding (time 0) was dramatically decreased (averaging 0.95 ± 0.29 μmol.g-1 liver ww, n = 5 in each group), without increase in NMR-visible Pi resonance. This value, also measured on extracts, was in agreement with the known decrease in hepatic ATP content in rats starved during a minimum of 24 hr [3538]. After force-feeding, total ATP liver content increased regularly (at a rate of 0.13 ± 0.01 μmol.h-1.g-1 liver ww, R2 = 0.92) until the 6th hr in the glucose group and until the 8th hour in the butyrate group. A positive and linear correlation between total ATP and glycogen contents was evidenced only during net glycogen synthesis (until 6 hr and 8 hr for glucose and butyrate groups, respectively). The net production of one μmol liver ATP (cytosolic and mitochondrial) was concomitant with a net glycogen synthesis of 92 and 80 μmol glucosyl units in the glucose and butyrate groups, respectively (Figure 4).

Figure 4
figure 4

Correlation between total ATP and glycogen contents during linear glycogen resynthesis. This linear step lasted until 6 hr and 8 hr in G and G+B groups, respectively. For each time post force-feeding, the glycogen content is expressed according to the total ATP content, the lowest values correspond to the shortest time and the highest values to the longest time. m ± SEM. n varied from 6 to 12 for each delay. Isocaloric (7.28 cal) force-feeding (expressed per g body weight) consisted of 18.2 mg glucose or 14 mg glucose + 1.9 mg butyrate.

Mitochondrial ATP production after force-feeding

In 48-hr starved rats, and immediately after force-feeding (0 hour), the rate of mitochondrial ATP synthesis (averaging 0.47 ± 0.12 μmol.min-1.g-1 liver ww (n = 3 in each group)), tended to be lower than in ad libitum fed rats: 0.92 ± 0.22 μmol.min-1.g-1 liver ww, n = 3). This is in agreement with a decrease in the content of the ATP synthase complex observed in liver of 18-hr starved rats [39].

After force-feeding with glucose alone, mitochondrial ATP turnover remained stable during the first 2 hr and then rapidly increased about two-fold at the 3rd hr (n = 10, P = 0.05 vs 2nd hr), the highest value being reached at the 6th hr. It decreased after the 6th hr to reach the basal level at the 10th hr (n = 9) (Figure 5).

Figure 5
figure 5

Effect of presence of butyrate in force-feeding bolus on time-course of mitochondrial ATP synthesis rate. m ± SEM. n varied from 6 to 12 for each delay. Isocaloric (7.28 cal) force-feeding (expressed per g body weight) consisted of 18.2 mg glucose or 14 mg glucose + 1.9 mg butyrate. Experimental conditions reflect the in vivo situation at each time of liver excision. Labeled means at a time without a common letter differ (t-test), P = 0.05. *Different from previous time within each group ≤0.05. **Different from 6th hour in Butyrate group, P < 0.04.

In the butyrate force-fed group, mitochondrial ATP turnover remained stable during the first 3 hr (0.79 ± 0.18 μmol.min-1.g-1 liver ww, n = 12), a value significantly lower than in the glucose group (n = 10) (P = 0.05). Then ATP turnover increased to 1.80 ± 0.33 μmol.min-1.g-1 liver ww at the 6th hr post force-feeding (n = 9, P = 0.01 vs 3rd hr). After that, turnover decreased until the 10th hr to a level similar to that observed in the glucose group (n = 9, P < 0.04 vs 6th hr).

The time constant (k) globally mimicked the changes in ATP turnover from the value of 0.53 ± 0.07 min-1 (n = 3) in starved rat at 0 hr feeding. At the 3rd hr, k was 1.55 ± 0.21 and 0.96 ± 0.19 min-1 in glucose (n = 10, P = 0.01 vs 0 hr) and glucose+butyrate (n = 12) groups, respectively. The maximal value in the butyrate group was obtained at the 6th hr (1.43 ± 0.31 min-1, n = 9, P = 0.05 vs 0 hour). At the 10th hr, k was 0.68 ± 0.08 and 0.51 ± 0.26 min-1 in glucose (n = 9) and glucose+butyrate (n = 9) groups, respectively.


Gut microbial activity can lead to the production of SCFAs such as butyrate. A hepatic butyrate removal close to 100% has been evidenced in rats and human [4, 5]. This raises the question of its effect in this central organ which is highly involved in carbohydrate metabolism.

Our aim was to simultaneously monitor by NMR spectroscopy in the whole rat liver (i) total ATP content and its unidirectional flux of mitochondrial synthesis and (ii) glycogen repletion, at different times following the force-feeding of rats with glucose alone or glucose plus butyrate.

In our experimental nutritional conditions after glucose uptake by the liver, glucose oxidation produced free energy, some being directly available for cellular activity and the rest for ATP storage. ATP is produced by glucose oxidation in two ways. First, it can be directly synthesized in cytosol via glycolysis, its end product, pyruvate, entering in the TCA cycle. Second, the reduced cofactors (NADH+H+, FADH2) resulting from the latter oxidative pathways are re-oxidized in mitochondria by the respiratory chain, ATP synthesis occurring by coupling between respiration and phosphorylative activity. Besides its oxidation, glucose leads to glycogen storage via glycogen synthesis. In this study, the liver glycogen content measured ex vivo reflected the in vivo balance between both glycogen synthesis and glycogen consumption. A net glycogen synthesis was observed 30 min after glucose bolus (data not shown), suggesting the rapid bioavailability of glucose. The glycogen storage in glycogen-depleted liver was linear during the first 6 hr after glucose-feeding. Total ATP content increased concomitantly with the progressive repletion of hepatic glycogen, thus suggesting a relationship between glycogen storage and a change in hepatic ATP content. The linear correlation between total ATP and glycogen contents was evidenced only during the step of net glycogen synthesis; the net production of one μmol liver ATP (cytosolic and mitochondrial) was concomitant with a net glycogen synthesis of 80-90 μmol glucosyl units. Glycogen synthesis needs UTP and then ATP, which must be supplied concomitantly. Since the cellular ATP content reflects the balance between the unidirectional fluxes of production and consumption at any given time, any increase in ATP content implies an increase in the rate of unidirectional fluxes of ATP synthesis. To our knowledge, this study is the first to monitor the evolution of the unidirectional flux of mitochondrial ATP supply in the whole liver after glucose feeding.

Although glycogen synthesis requires energy, the total ATP content increased concomitantly with the glycogen content whereas the mitochondrial ATP supply remained stable during the first 2 hr. One ATP was needed to phosphorylate the exogenous glucose, and one UTP (then one ATP) was used to condense one glucosyl unit arising from glucose-1-phosphate. The rate of net glycogen synthesis averaged 11 μ liver ww theoretically requiring 22 μ corresponding to 50% of the measured basal flux of mitochondrial ATP production (42 μ liver ww), the other 50% part contributing to maintain the basal cellular activities namely ionic homeostasis [40, 41] and micro tubular migration [41]. In our conditions of totally depleted glycogen the linear increase in total ATP content thus implies another source that can be only the glycolytic pathway from exogenous glucose. The direct evidence of net glycogen synthesis and indirect demonstration of glycolysis lead to postulate for the establishment of a glucose cycling, this cycling being previously described [12, 15].

During the initial phase of glycogen repletion, the stored polymer content was likely not high enough to contribute to glycogenolysis or subsequent cycling of glycogen. Any cycling of glycogen [4244] could predominate only when the ATP furniture is sufficient and when the hepatic glycogen store becomes sufficient (after 2 hr). This is in agreement with the previous described absence of indirect glycogen synthesis for 2 hours after glucose feeding in rat [44]. Since the net rate of glycogen synthesis was constant, any onset of unidirectional glycogenolysis flux must be algebraically compensated by an increase in the unidirectional flux of glycogen synthesis with the same magnitude. Since an increase in this synthesis flux needs an increase in UTP supply, the increase in the ATP turnover observed at the 3rd hour could be related to this process. This hypothesis of late glycogen cycling is supported by the evidence of progressive glycogenolysis during ex vivo incorporation of enriched glucose in newly synthesized glycogen in the isolated perfused liver of fasting rats [45]. Thereafter, a decrease in the unidirectional flux of glycogen synthesis is evidenced by the plateau (and the subsequent decrease) of glycogen content observed after 6 hr post-feeding, and may be related in part to the decreased bioavailability of the exogenous glucose. The progressive decrease in mitochondrial ATP turnover could be partly due to the decrease in energy need related to the reduction in unidirectional flux of glycogen synthesis.

Although the linear correlation between total ATP and glycogen contents was not affected by ingested butyrate, the step of net glycogen synthesis and total ATP synthesis was extended from the 6th to the 8th hour post-feeding. Consequently butyrate might delay the establishment of glycogenolysis as it is an energy substrate. In a previous NMR study using 13C-glucose enrichment on ex vivo perfused liver isolated from fasted rats, we demonstrated a decrease in the rate of glycogenolysis flux in the butyrate+glucose perfused group compared to an isocaloric perfusion of glucose alone [46]. Partial and direct inhibition of phosphorylase activity by butyrate cannot be excluded but remains to be demonstrated. This result reinforces the hypothesis that butyrate may delay the establishment of glycogenolysis.

In another study [33], we showed that one of the hepatic effects of butyrate in vivo, as other FFA, is glucose-sparing, which is due to a preferential butyrate oxidation concomitant with glycolysis slow-down [47], promoting glucose disposal in the glycogen synthesis pathway during the post-prandial state. Unlike glucose oxidation, butyrate oxidation leads via mitochondrial -oxidation to the production of acetyl CoA. Acetyl CoA is then oxidized via the TCA cycle and impairs pyruvate oxidation, the end-product of aerobic glycolysis. The rate of glycolysis and its cytosolic ATP production are thus reduced.

For the first time, we demonstrate that butyrate ingestion delays from the third hour to 6th hour the increase in the unidirectional flux of mitochondrial ATP supply. Since butyrate oxidation is known to increase liver respiration [8], a stimulation of mitochondrial ATP production could be expected rapidly after bolus feeding. However, a controlling and inhibiting effect of butyrate per se was previously described in the isolated perfused rat liver, leading to reduced mitochondrial phosphorylation activity and a large decrease in oxidative phosphorylation yield [9]. As long as butyrate bioavailability and its oxidation were maintained, the onset of glycolysis and the stimulation of the unidirectional flux of mitochondrial ATP supply were delayed.


The strength of this study is that it is the first to measure simultaneously the hepatic changes of glycogen repletion and the rate of mitochondrial ATP synthesis after feeding of 48 hr starved rats in an experimental condition reflecting in vivo changes. The main results are the evidence of (i) a linear relation between total ATP and glycogen contents only during the net glycogen synthesis, (ii) a late and transient increase in rate of mitochondrial ATP supply probably linked to glycogen metabolism and (iii) that butyrate delayed this late and transient increase of ATP turnover. Whether these phenomena are dependent on insulin remains to be investigated.



adenosine triphosphate


free fatty acids




Krebs-Henseleit buffer


time constant


nuclear magnetic resonance


ATP turnover


short-chain free fatty acids


wet weight.


  1. Nyman M, Asp NG, Cummings J, Wiggins H: Fermentation of dietary fiber in the intestinal tract: comparison between man and rat. Br J Nutr. 1986, 55: 487-96. 10.1079/BJN19860056.

    Article  CAS  Google Scholar 

  2. Candela M, Maccaferri S, Turroni S, Carnevali P, Brigidi P: Functional intestinal microbiome, new frontiers in prebiotic design. Int J Food Microbiol. 2010, 140: 93-101. 10.1016/j.ijfoodmicro.2010.04.017.

    Article  CAS  Google Scholar 

  3. Cani PD, Delzenne NM, Amar J, Burcelin R: Role of gut microflora in the development of obesity and insulin resistance following high-fat diet feeding. Pathol Biol (Paris). 2008, 56: 305-9.

    Article  CAS  Google Scholar 

  4. Demigné C, Yacoub C, Rémésy C: Effects of absorption of large amounts of volatile fatty acids on rat liver metabolism. J Nutr. 1986, 116: 77-86.

    Google Scholar 

  5. Bloemen JG, Venema K, van de Poll MC, Olde Damink SW, Buurman WA, Dejong CH: Short chain fatty acids exchange across the gut and liver in humans measured at surgery. Clin Nutr. 2009, 28: 657-61. 10.1016/j.clnu.2009.05.011.

    Article  CAS  Google Scholar 

  6. Schönfeld P, Schild L, Kunz W: Long-chain fatty acids act as a protonophoric uncouplers of oxidative phosphorylation in rat liver mitochondria. Biochim Biophys Acta. 1989, 977: 266-72. 10.1016/S0005-2728(89)80080-5.

    Article  Google Scholar 

  7. Mokhova EN, Khailova LS: Involvement of mitochondrial inner membrane anion carriers in the uncoupling effect of fatty acids. Biochemistry. 2005, 70: 159-63.

    CAS  Google Scholar 

  8. Beauvieux MC, Tissier P, Gin H, Canioni P, Gallis JL: Butyrate impairs energy metabolism in isolated perfused liver of fed rats. J Nutr. 2001, 131: 1986-92.

    CAS  Google Scholar 

  9. Gallis JL, Tissier P, Gin H, Beauvieux MC: Decrease in oxidative phosphorylation yield in presence of butyrate in perfused liver isolated from fed rats. BMC Physiol. 2007, 7: 8-10.1186/1472-6793-7-8.

    Article  Google Scholar 

  10. Baillet-Blanco L, Beauvieux MC, Gin H, Rigalleau V, Gallis JL: Insulin induces a positive relationship between the rates of ATP and glycogen changes in isolated rat liver in presence of glucose; a 31P and 13C NMR study. Nutr Metab. 2005, 2: 32-10.1186/1743-7075-2-32.

    Article  Google Scholar 

  11. Landau BR, Wahren J: Quantification of the pathways followed in hepatic glycogen formation from glucose. FASEB J. 1988, 2: 1368-75.

    Google Scholar 

  12. Gustafson LA, Neeft M, Reijngoud DJ, Kuipers F, Sauerwein HP, Romijn JA, Herling AW, Burger HJ, Meijer AJ: Fatty acid and amino acid modulation of glucose cycling in isolated rat hepatocytes. Biochem J. 2001, 358: 665-71. 10.1042/0264-6021:3580665.

    Article  CAS  Google Scholar 

  13. Chen X, Iqbal N, Boden G: The effects of free fatty acids on gluconeogenesis and glycogenolysis in normal subjects. J Clin Invest. 1999, 103: 365-72. 10.1172/JCI5479.

    Article  CAS  Google Scholar 

  14. Sprangers F, Rominj JA, Endert E, Ackermans MT, Sauerwein HP: The role of free fatty acids (FFA) in the regulation of intrahepatic fluxes of glucose and glycogen metabolism during short-term starvation in healthy volunteers. Clin Nutr. 2001, 20: 177-9. 10.1054/clnu.2000.0372.

    Article  CAS  Google Scholar 

  15. Boden G, Chen X, Capulong E, Mozzoli M: Effects of free fatty acids on gluconeogenesis and autoregulation of glucose production in type 2 diabetes. Diabetes. 2001, 50: 810-6. 10.2337/diabetes.50.4.810.

    Article  CAS  Google Scholar 

  16. Künnecke B, Seeling J: Glycogen metabolism as detected by in vivo and in vitro 13C-NMR spectroscopy using [1,2-13C2]glucose as substrate. Biochim Biophys Acta. 1991, 1095: 103-13. 10.1016/0167-4889(91)90071-5.

    Article  Google Scholar 

  17. Schmid AI, Chmelík M, Szendroedi J, Krssák M, Brehm A, Moser E, Roden M: Quantitative ATP synthesis in human liver measured by localized 31P spectroscopy using the magnetization transfer experiment. NMR Biomed. 2008, 21: 437-43. 10.1002/nbm.1207.

    Article  CAS  Google Scholar 

  18. Ikai I, Okuda M, Doliba N, Chance B: Rate of ATP synthesis in the perfused rat liver by 31P cryo-NMR. J Biol Chem. 1990, 265: 22097-100.

    CAS  Google Scholar 

  19. Beauvieux MC, Stephant A, Serhan N, Gin H, Couzigou P, Gallis JL: Resveratrol counteracts ethanol effect by stimulating glycolysis: NMR studies with chemical inhibitor or magnetization transfer in isolated perfused rat liver [abstract]. Gut. 2010, 59: A211-

    Google Scholar 

  20. Thoma WJ, Ugurbil K: Saturation-transfer studies of ATP-Pi exchange in isolated perfused rat liver. Biochim Biophys Acta. 1987, 893: 225-31. 10.1016/0005-2728(87)90043-0.

    Article  CAS  Google Scholar 

  21. Beauvieux MC, Tissier P, Couzigou P, Gin H, Gallis JL: Ethanol perfusion increases the yield of oxidative phosphorylation in isolated liver of fed rats. Biochim Biophys Acta. 2002, 1570: 135-40.

    Article  CAS  Google Scholar 

  22. Guignot L, Mithieux G: Mechanisms by which insulin, associated or not with glucose, may inhibit hepatic glucose production in the rat. Am J Physiol. 1999, 277: E984-89.

    CAS  Google Scholar 

  23. Anand P, Gruppuso PA: The regulation of hepatic protein synthesis during fasting in the rat. J Biol Chem. 2005, 280: 16427-436. 10.1074/jbc.M410576200.

    Article  CAS  Google Scholar 

  24. Gautier-Stein A, Zitoun C, Lalli E, Mithieux G, Rajas F: Transcriptional regulation of the glucose-6-phosphatase gene by cAMP/vasoactive intestinal peptide in the intestine. Role of HNF4alpha, CREM, HNF1alpha, and C/EBPalpha. J Biol Chem. 2006, 280: 31268-278.

    Article  Google Scholar 

  25. Nanji AA, French SW: Animal models of alcoholic liver disease; focus on the intragastric feeding model. Alcohol Res Health. 2003, 27: 325-30.

    Google Scholar 

  26. Poole TEP: UFAW guidelines handbook on the care and management of laboratory animals. 1999, Terrestrial vertebrates ed Oxford

    Google Scholar 

  27. Exton JH: The perfused rat liver. Methods Enzymol. 1975, 39: 25-36.

    Article  CAS  Google Scholar 

  28. Cohen SM: Simultaneous 13C and 31P NMR studies of perfused rat liver. Effects of insulin and glucagon and a 13C NMR assay of free Mg2+. J Biol Chem. 1983, 258: 14294-308.

    CAS  Google Scholar 

  29. Desmoulin F, Canioni P, Crotte C, Gérolami A, Cozzone PJ: Hepatic metabolism during acute ethanol administration: a phosphorus-31 nuclear magnetic resonance study on the perfused rat liver under normoxic or hypoxic conditions. Hepatology. 1987, 7: 315-23. 10.1002/hep.1840070217.

    Article  CAS  Google Scholar 

  30. Delmas-Beauvieux MC, Gallis JL, Rousse N, Clerc M, Canioni P: Phosphorus-31 nuclear magnetic resonance of isolated rat liver during hypothermic ischemia and subsequent normothermic perfusion. J Hepatol. 1992, 15: 192-201. 10.1016/0168-8278(92)90035-N.

    Article  CAS  Google Scholar 

  31. Cardin S, Emshwiller M, Jackson PA, Snead WL, Hastings J, Edgerton DS, Cherrington AD: Portal glucose infusion increases hepatic glycogen deposition in conscious unrestrained rats. J Appl Physiol. 1999, 87: 1470-75.

    CAS  Google Scholar 

  32. Shulman GI, Rothman DL, Chung Y, Rossetti L, Petit WA, Barrett EJ, Shulman RG: 13C NMR studies of glycogen turnover in the perfused rat liver. J Biol Chem. 1988, 263: 5027-9.

    CAS  Google Scholar 

  33. Beauvieux MC, Roumes H, Robert N, Gin H, Rigalleau V, Gallis JL: Butyrate ingestion improves hepatic glycogen storage in the re-fed rat. BMC Physiol. 2008, 8: 19-10.1186/1472-6793-8-19.

    Article  Google Scholar 

  34. Gallis JL, Delmas-Beauvieux MC, Biran M, Rousse N, Durand T, Canioni P: Is cellular integrity responsible for the partial NMR-invisibility of ATP in isolated rat liver?. NMR Biomed. 1991, 4: 279-85. 10.1002/nbm.1940040606.

    Article  CAS  Google Scholar 

  35. Start C, Newsholme EA: The effects of starvation and alloxan-diabetes on the contents of citrate and other metabolic intermediates in rat liver. Biochem J. 1968, 107: 411-15.

    Article  CAS  Google Scholar 

  36. Jennische E: Effects of ischemia on the hepatic cell membrane potential in the rat. Difference between fed and fasted animals. Acta Physiol Scand. 1983, 118: 69-73. 10.1111/j.1748-1716.1983.tb07242.x.

    Article  CAS  Google Scholar 

  37. Acco A, Comar JF, Bracht A: Metabolic effects of Propofol in the isolated perfused rat liver. Basic Clin Pharmacol Toxicol. 2004, 95: 166-74.

    Article  CAS  Google Scholar 

  38. Silva FM, da Silva MH, Bracht A, Eller GJ, Constantin RP, Yamamoto NS: Effects of metformin on glucose metabolism of perfused rat livers. Mol Cell Biochem. 2010, 340: 283-89. 10.1007/s11010-010-0429-2.

    Article  CAS  Google Scholar 

  39. Vendemiale G, Grattagliano I, Caraceni P, Caraccio G, Domenicali M, Dall'Agata M, Trevisani F, Guerrieri F, Bernardi M, Altomare E: Mitochondrial oxidative injury and energy metabolism alteration in rat fatty liver: effect of the nutritional status. Hepatology. 2001, 33: 808-815. 10.1053/jhep.2001.23060.

    Article  CAS  Google Scholar 

  40. Chinet AE: Composantes et contrôle de la dépense énergétique cellulaire chez l'endotherme. Reprod Nutr Dev. 1990, 30: 1-11. 10.1051/rnd:19900101.

    Article  CAS  Google Scholar 

  41. Groen AK: Control of mitochondrial oxidative phosphorylation in the intact cell. Quantification of control in studies on intermediary metabolism. Edited by: Kanters BV. 1984, 61-66.

    Google Scholar 

  42. Kurland IJ, Pilkis SJ: Indirect versus direct routes of hepatic glycogen synthesis. FASEB J. 1989, 3: 2277-81.

    CAS  Google Scholar 

  43. Shulman GI, Rothman DL, Smith D, Johnson CM, Blair JB, Shulman RG, DeFronzo RA: Mechanism of liver glycogen repletion in vivo by nuclear magnetic resonance spectroscopy. J Clin Invest. 1985, 76: 1229-36. 10.1172/JCI112078.

    Article  CAS  Google Scholar 

  44. Huang MT, Veech RL: Role of the direct and indirect pathways for glycogen synthesis in rat liver in the post prandial state. J Clin Invest. 1988, 81: 872-78. 10.1172/JCI113397.

    Article  CAS  Google Scholar 

  45. Beauvieux MC, Couzigou P, Roumes H, Rigalleau V, Gin H, Gallis JL: Moderate ethanol supply inhibits both glycogen synthesis and glycogenolysis in the perfused and isolated rat liver [abstract]. Proc Nutr Soc. 2008, 67 (OCE): E198-

    Article  Google Scholar 

  46. Beauvieux MC, Roumes H, Rigalleau V, Gin H, Gallis JL: Le butyrate favorise l'accumulation de glycogène hépatique en inhibant la glycogénolyse: étude par résonance magnétique nucléaire du P31 dans le foie de rat isolé et perfusé [abstract]. Nutr Clin Métab. 2006, 20: S103-

    Google Scholar 

  47. Hue L, Taegtmeyer H: The Randle cycle revisited: a new head for an old hat. Am J Physiol Endocrinol Metab. 2009, 297: E578-91. 10.1152/ajpendo.00093.2009.

    Article  CAS  Google Scholar 

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We thank Ray Cooke for language editing.

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Correspondence to Marie-Christine Beauvieux.

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JLG designed and conducted the research, analyzed the data and wrote the paper, HR performed the experiments and analyzed the data, HG wrote the paper, MCB conducted the research and wrote the paper. All authors read and approved the final manuscript.

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Gallis, JL., Gin, H., Roumes, H. et al. A metabolic link between mitochondrial ATP synthesis and liver glycogen metabolism: NMR study in rats re-fed with butyrate and/or glucose. Nutr Metab (Lond) 8, 38 (2011).

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