Resistance training to improve type 2 diabetes: working toward a prescription for the future
- Dominik H. Pesta1, 2, 3, 4Email author,
- Renata L. S. Goncalves5,
- Anila K. Madiraju6,
- Barbara Strasser†7 and
- Lauren M. Sparks†8, 9Email author
© The Author(s). 2017
Received: 6 October 2016
Accepted: 14 February 2017
Published: 2 March 2017
The prevalence of type 2 diabetes (T2D) is rapidly increasing, and effective strategies to manage and prevent this disease are urgently needed. Resistance training (RT) promotes health benefits through increased skeletal muscle mass and qualitative adaptations, such as enhanced glucose transport and mitochondrial oxidative capacity. In particular, mitochondrial adaptations triggered by RT provide evidence for this type of exercise as a feasible lifestyle recommendation to combat T2D, a disease typically characterized by altered muscle mitochondrial function. Recently, the synergistic and antagonistic effects of combined training and Metformin use have come into question and warrant more in-depth prospective investigations. In the future, clinical intervention studies should elucidate the mechanisms driving RT-mitigated mitochondrial adaptations in muscle and their link to improvements in glycemic control, cholesterol metabolism and other cardiovascular disease risk factors in individuals with T2D.
KeywordsResistance training Type 2 diabetes Skeletal muscle Mitochondrial function
The significance of resistance training for individuals with type 2 diabetes: moving beyond what we already know
The prevalence of type 2 diabetes (T2D) continues to increase. Within the next 20 years, the number of people affected by this disease is expected to reach almost 600 million worldwide . T2D is accompanied by a host of risk factors including dyslipidemia, hypertension and cardiovascular disease , thus putting a severe burden on our global health care systems. Apart from medication, chronic exercise (i.e. systematic training performed repeatedly) is a proven prevention and treatment strategy for individuals with pre-diabetes and T2D [3–5]. Recent reviews and meta-analyses, including the 2016 joint position statement on physical activity and T2D from the American Diabetes Association , have highlighted the beneficial effects of chronic endurance training (ET), resistance training (RT) and/or combined (ET + RT) interventions for ameliorating insulin sensitivity and glycemic control in individuals with T2D [7, 8]. Chronic ET alone has a well-established role in enhancing insulin sensitivity via glucose uptake and disposal (reviewed in ) and in increasing muscle mitochondrial density and oxidative capacity . A limited number of studies have demonstrated that chronic RT alone enhances glycemic control [11, 12] and muscle substrate metabolism in individuals with T2D , yet the underlying mechanisms inducing these health benefits, particularly those related to muscle mitochondrial function, remain to be elucidated.
The present review focuses on the effects of chronic RT on glycemic control, substrate metabolism and the molecular mechanisms that may influence these adaptations in individuals with T2D. We place a special emphasis on skeletal muscle, the interaction between anti-diabetic medication use and training stimulus, and incorporate adipose tissue as another significant target organ for RT-mediated metabolic adaptations in T2D. Since little is known about the independent effects of chronic RT on mitochondrial adaptations in skeletal muscle and adipose tissue of individuals with T2D, we identify gaps in the current literature and raise important questions that future RT-focused trials in individuals with T2D will hopefully address. Improving our understanding of the mechanisms underpinning chronic RT-mitigated metabolic adaptions in T2D will move the scientific community (researchers and clinicians alike) beyond what we already know and toward future investigations focused on molecular determinants of the individual training responses in T2D.
Chronic resistance training effects on muscle mass, fiber type and glycemic control
Resistance training-induced gains in muscle mass are not solely responsible for improved muscle substrate metabolism in type 2 diabetes
Metabolic adaptations within skeletal muscle in response to resistance training: how much does fiber type matter?
Type IIx fibers are described as having a glycolytic phenotype, relying on glucose more than fat as a fuel, and facilitating short-duration anaerobic activities. It has been shown that Type IIx fibers are present in a higher proportion in individuals with T2D . Hyperinsulinemia—a hallmark feature of insulin resistance and T2D—can shift muscle fiber type toward a glycolytic phenotype by increasing Type IIx myosin heavy chain gene expression . Physical inactivity and immobilization have similar effects on fiber type shift . Interestingly, first-degree relatives of individuals with T2D have a ~30% higher proportion of Type IIx fibers than individuals without a family history of T2D. Type IIx fiber content was also negatively associated with glucose disposal rates in these same individuals . Paradoxically, elite strength and speed athletes have a high proportion of Type IIx fibers and are metabolically healthy, yet the high proportion of Type IIx fibers observed in individuals with T2D is concomitant with overall blunted substrate oxidation and appears to be less advantageous for these individuals. It is tempting to speculate that the high number of Type IIx fibers in individuals with T2D could be “trained” to utilize fuel more effectively as observed in strength-based athletes. Four to six weeks of moderate intensity RT (at 40–50% of the one-repetition maximum, 1RM) markedly increased skeletal muscle glucose uptake in non-obese individuals with T2D , which was largely attributed to a shift in fiber type toward Type IIa fibers. Single fiber analysis revealed that Type IIa fibers were the ones with the highest glucose uptake and GLUT4 content among the Type II fiber population [25, 26]. Type IIa fibers also had a higher capillary density and showed a greater insulin response than Type IIx fibers . Although Type IIa fibers exhibited more marked glycogen depletion during an exercise bout and faster glycogen re-synthesis following the exercise bout , it remains to be determined whether altering fiber type distribution benefits individuals with T2D in this respect. It is entirely possible that fiber type composition is irrelevant if the cellular metabolic machinery (e.g., glucose transport, mitochondria, etc.) is dysfunctional. In other words, quantity does not necessarily equal quality. Challenging the idea that switching fiber type confers a metabolic advantage, two independent studies demonstrated that chronic RT-driven improvements in insulin responsiveness and high-density lipoprotein (HDL) levels in individuals with T2D occurred without any changes in fiber type composition [29, 30], a phenomenon routinely observed in healthy individuals . Metabolic adaptations within muscle can therefore occur independently of a change in muscle fiber type composition. It is important to note that direct comparisons of the effects of different durations and intensities of RT on fiber type composition are virtually absent from the literature.
Chronic resistance training and mitochondrial fitness
Resistance training effects on muscle mitochondrial function in individuals with type 2 diabetes: how much do we really know?
Perturbations in mitochondrial oxidative capacity play a major role in the development and progression of insulin resistance and T2D . As few as 3 days of high-fat feeding are sufficient to induce insulin resistance and reduce muscle mitochondrial oxidative phosphorylation at the transcriptional level in lean, sedentary individuals . Individuals with T2D have reduced mitochondrial oxidative capacity when expressed per unit of muscle mass compared to healthy individuals; however, when mitochondrial oxidative capacity is normalized to markers of mitochondrial content (e.g., mitochondrial DNA copy number, citrate synthase activity), these differences are insignificant [16, 34]. That being said, disrupted mitochondrial morphology and a 35% reduction in mitochondrial size reported in individuals with T2D are indicative of functional impairment .
Aerobic and combined training improve mitochondrial function . Mechanical stress induces activation of the mitogen-activated protein kinase (MAPK) family of proteins (extracellular-regulated kinase (ERK1/2) and p38 MAPK). Activation of p38 MAPK during contraction stimulates activating transcription factor (ATF) 2 and MEF2, which increase PGC-1α expression and improve mitochondrial function in skeletal muscle (Fig. 1) . Some evidence to support a role for chronic RT in increasing muscle mitochondrial oxidative capacity exists in healthy individuals [37–39]; however, similar data in individuals with T2D are limited. RT (alone or in combination with ET) has only recently been recognized as a promising intervention to maintain muscle mitochondrial oxidative capacity  and improve the overall metabolic phenotype of individuals with T2D [13, 41]. Discrepancies in the measured outcomes and the details of the training protocols implemented in RT interventions have made it difficult to ascertain the value of implementing chronic RT to improve muscle mitochondrial function in individuals with T2D.
One interesting theory on how to improve muscle mitochondrial oxidative capacity in diabetes is via ‘gene shifting’. A recent report suggests that satellite cell activation following chronic RT leads to incorporation of new mitochondrial DNA into mature muscle cells . This phenomenon, known as ‘gene shifting’, may help normalize mitochondrial oxidative capacity in individuals suffering from impaired mitochondrial function by providing undamaged mitochondrial DNA from which new, functional mitochondrial proteins can be synthesized. In support of this hypothesis, a supervised 14-week RT regimen (three times per week at 50–80% of the 1RM) increased cytochrome c oxidase (COX) activity, enhanced mitochondrial creatine kinase levels and decreased oxidative DNA damage in elderly men and women . These findings suggest that chronic RT up-regulates selected components of the mitochondrial electron transport system which may convey many of the beneficial effects of this exercise modality .
Collectively, these findings suggest that chronic RT is capable of inducing muscle mitochondrial biogenesis and enhancing downstream oxidative capacity. In addition to biogenesis, mitochondria also undergo remodeling (i.e., fusion and fission) to enhance and/or preserve function. RT can act as a trigger to induce mitochondrial fission, followed by recovery-induced fusion and mitochondrial biogenesis. These RT-triggered processes may act as natural selection at the level of the mitochondrion (mito-checkpoint) and eliminate disadvantageous mitochondria, thereby driving adaptive selection of advantageous phenotypic variations . This concept adds another dimension to the current understanding of chronic RT-mitigated improvements in muscle mitochondrial health.
Resistance training-induced oxidative stress in type 2 diabetes: is defense the best offense?
T2D is characterized by excessive reactive oxygen species (ROS) emission which presumably leads to oxidative stress in different tissues [52, 53]. There are at least 11 sites in muscle mitochondria capable of producing ROS and their rates under different (patho)physiological conditions still need to be determined . Recent estimations assessing ex vivo mitochondrial superoxide/H2O2 production in conditions mimicking physiological states have reported that 0.35% of oxygen consumed at rest is diverted to ROS production, and that this proportion is reduced to 0.03–0.01% during exercise . Given that mitochondria superoxide/H2O2 production is likely to decrease during moderate exercise, it is important to also consider other cellular reactions and sites as significant mediators of ROS production. Both ET and RT increase oxidant production, which can lead to oxidative stress [56, 57]. ROS can activate c-jun N-terminal kinase (JNK) signaling as evidenced by a recent report demonstrating that infusion of the antioxidant N-acetylcysteine reduced JNK signaling during aerobic exercise training  (Fig. 1). Interleukin-6 (IL-6) is associated with a variety of metabolic effects across different organs and its secretion during contraction is JNK-dependent , emphasizing the importance of JNK signaling and ROS-mediated JNK activation in facilitating metabolic adaptations to aerobic exercise training . In a comparative study assessing the effects of exhaustive ET vs. RT on ROS production, both training modalities increased oxidative stress; however, lipids were affected by peroxidation only during RT, and proteins were oxidized and formed carbonyls during ET . Training frequency also impacts ROS production and oxidative stress. While a single RT session was sufficient to induce oxidative damage in untrained men , 6 months of RT (three times per week at 50–80% of the 1RM) induced whole-body adaptations resulting in decreased training-induced oxidative stress and homocysteine levels . Data on RT-mediated oxidative stress are sparse in individuals with T2D. A recent study reported that 12 months of supervised ET, RT and flexibility training equally reduced oxidative stress in men with T2D .
In addition to promoting acute ROS formation , aerobic exercise training generally improves muscle antioxidant defense mechanisms . Interestingly, mitochondrial hydrogen peroxide (H2O2) production is important for muscle differentiation in vitro, and mitochondria-targeted antioxidant treatment with MitoQ and MitoTEMPOL, as well as mitochondria-targeted catalase, blocks this effect . As such, acute ROS formation during RT may also have beneficial effects on muscle differentiation in vivo. Indeed, mitochondrial ROS generation might be involved in initiating mitochondrial biogenesis . Transient ROS “build-up” has been suggested to prime the cellular redox system, which culminates in an improved handling of subsequent pro-oxidant environments. Oxidative stress must therefore be considered in connection with radical scavenging activities and cellular antioxidant defense mechanisms. A 12-week RT intervention (twice per week at 50–75% of the 1RM) not only reduced oxidative stress, but also significantly increased cytosolic and mitochondrial antioxidant proteins [superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (GPX1), peroxiredoxin isoform 5 (PRDX5), heat-shock-protein-70 (HSP70)] in muscle of individuals with T2D .
Chronic RT effects on ROS production in human muscle are relatively unexplored. Due to the absence of reliable techniques to precisely measure ROS production in vivo, fundamental questions regarding the sources of ROS and the effects of training modalities on ROS signaling remain unanswered. As such, more studies are required to elucidate how ROS affects the hypertrophic and adaptive responses of human muscle to chronic RT, especially in T2D. Unraveling these pathways could lead to a better understanding of the potential therapeutic role of antioxidant supplements in treating metabolic impairments. Furthermore, it is important to consider the interaction of medication or supplement use with the antioxidant defense system (Fig. 2). A recent review explored the novel hypothesis that attenuation of oxidative stress from exercise training by these exogenous compounds blunts beneficial metabolic adaptations .
Chronic resistance training and medication use
Metformin and exercise training in diabetes: the good, the bad and the unknown
In recent years, the combined effects of metformin treatment and training regimens on metabolic outcomes have gained attention, the majority of these studies conducted in healthy and/or pre-diabetic populations [69–73] (Fig. 2). Metformin is the most widely prescribed first-line oral anti-diabetic drug recommended by the American Diabetes Association (ADA). The exact molecular mechanism of action underlying this drug’s physiological benefits remains mysterious, although it is often prescribed together with a regular exercise training program as part of a lifestyle intervention. Metformin is known to have pleiotropic effects and multiple potential target pathways. One possible site of metformin action is inhibition of mitochondrial respiratory system complex I, increasing the [AMP]/[ATP] ratio (much like muscle contraction does) and activating AMPK. AMPK phosphorylation of multiple downstream effectors can divert cellular metabolism towards restoration of energy homeostasis , thereby improving insulin sensitivity (Fig. 2). ET-mediated increases in insulin sensitivity also act in part through AMPK activation , suggesting that exercise training and metformin could have additive effects. In support, a recent randomized, controlled chronic exercise training (ET, RT, ET + RT) intervention (22 weeks) in individuals with T2D demonstrated that compared with controls, ET led to a significant reduction in HbA1c in metformin users (-0.57%) but not in untreated individuals (-0.17%). However, metformin did not have any effect on improvements in indicators of aerobic fitness, strength, body weight or waist circumference . Importantly, this effect was only observed with ET and ET + RT. The RT only group did not achieve significant reductions in HbA1c (with or without metformin) compared with the control group.
Alternatively, metformin was also documented to have opposing effects on fat oxidation during and following a single bout of ET in healthy individuals , and a recent report demonstrated that metformin blunted the full effect of 12 weeks of combined ET + RT on insulin sensitivity by 25–30% in glucose intolerant individuals . This same investigation demonstrated that metformin use blunted some of the beneficial effects of the combined training related to a reduction in cardiovascular disease risk such as systolic blood pressure or high sensitivity C-reactive protein (hs-CRP) . These findings conflict with an earlier investigation in insulin-resistant individuals in which two to three weeks of metformin treatment blunted the effects of a single bout of ET on insulin sensitivity . The above studies suggest some positive and negative interactions between metformin use and adaptations to both single and repeated bouts of ET and combined ET + RT on glycemic control. Taken together, these data suggest that when exercise training is implemented in a population of current metformin users, which is commonplace in a clinical setting, it is important to consider how use of this medication may affect an individual’s training response in terms of glycemic control.
Biguanides such as metformin have also been recently identified as a new class of mitochondrial glycerophosphate dehydrogenase (mGPD)  and ATP synthase inhibitors . Inhibition of ATP synthase may affect energetics in skeletal muscle and recovery of energy charge during exercise. Inhibition of mGPD by metformin leading to decreased cytosolic [NAD+]/[NADH] may alter activity of SIRT1, decreasing deacetylation of PGC-1α and reversing potential positive impact of exercise on mitochondrial biogenesis. Also relevant is the recent finding that metformin’s effect as a cancer therapeutic is partially mediated by inhibition of mTOR, raising the question of whether metformin may influence the positive effects of Akt-mTOR pathway activation on muscle mass . It remains to be determined how a chronic RT regimen would impact individuals with T2D currently taking metformin and other anti-diabetic drugs (Fig. 2). More research on the detailed effects of metformin on adaptations to ET and RT, especially in the context of varying intensities and duration, is warranted. Furthermore, the significance of metformin effects directly in the muscle at prescribed doses requires attention, as it is thought that metformin primarily targets liver metabolism. It is possible that metformin-mediated amelioration of whole-body glucose homeostasis via inhibition of uncontrolled liver gluconeogenesis may be enough to restore muscle insulin sensitivity and therefore promote beneficial effects of exercise training in the muscle. Based on current evidence, we speculate that in the muscle, anti-diabetic drugs such as metformin – due to inhibition of complex I of the electron transport system and subsequent AMPK activation – may interfere with adaptations to ET to a greater extent than RT, which recruits a different signaling cascade than AMPK. This could possibly establish RT as a reasonable training modality for a cohort taking metformin, particularly those individuals with T2D.
Chronic resistance training and adipose tissue
Adipose tissue is an important (and understudied) target organ of resistance training in diabetes
Studies of exercise training, chronic RT in particular, on white adipose tissue (WAT) remodeling in humans are sparse. WAT secretes two major pro-inflammatory cytokines: interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Obesity is characterized by a state of chronic low-grade inflammation, which is implicated in the pathogenesis of T2D , and circulating levels of both IL-6 and TNF-α are inversely related to glycemic control and insulin sensitivity . Modulation of low-grade inflammation by 12 weeks of ET (40 minutes at 60–80% of the maximal heart rate) and RT (three times per week at 60–80% of the 1RM) was investigated in obese individuals with T2D. Although ET was more effective in reducing adipocytokines in response to the chronic training, RT also significantly reduced circulating levels of TNF-α and IL-6 . RT for 16 weeks (three times per week at 60–85% of the 1RM) in obese adolescents significantly reduced IL-6 and TNF-α plasma levels, and changes in muscle strength were directly related to changes in pro-inflammatory cytokines . These data suggest that chronic RT induces a signaling pathway that alleviates systemic inflammation. In another study, 12 weeks of supervised RT (3 days per week) in individuals with T2D decreased plasma levels of hs-CRP, a non-specific marker of inflammation, and increased the beneficial adipokine Visfatin . According to some studies [85, 86] excess post-exercise oxygen consumption is higher after RT than after ET. This phase is characterized by utilization of fat as a fuel, which could benefit weight loss . Although data are limited, collectively, these studies highlight the systemic anti-inflammatory effects of RT (presumably via WAT) and the potential of chronic RT to improve body composition and alleviate chronic low-grade inflammation associated with obesity and T2D. How chronic RT affects WAT function (e.g., fibrosis, angiogenesis, browning, etc.) and signaling pathways within the organ itself are areas of research that require greater attention and could shed light on exercise training targets that elicit a positive metabolic response in individuals with T2D.
Future directions – toward personalized medicine?
Due to the large inherent variability of responses to the same RT program, general recommendations are less than ideal. The future of T2D research is moving in the direction of personalized medicine. Current research in this area continues to discover signaling pathways that differ even amongst the most homogenous groups of individuals, and these differences ultimately lead to variations in their physiological responses to medications and treatments. It is imperative that we exploit these inter-individual responses following exercise training interventions in individuals with T2D to maximize each person’s beneficial adaptation to a training program. A clear distinction of the different types of RT will be necessary for the implementation of exercise training as a feasible lifestyle modification in light of current and future progression toward personalized medicine. Different RT programs (with varying intensities) will lead to diverse results and metabolic outcomes, and this cause-effect relationship must be clearly established for RT in order to maximize the benefits for individuals with T2D. In this effort, more research comparing supervised ET interventions with RT interventions of varying intensities, durations and volumes including long-term training studies using different modes of periodization, is required. This will further elucidate exercise-mediated effects on whole-body metabolism and muscle mitochondrial function, specifically in individuals with T2D. In addition, the effects of RT on other major target organs, such as WAT, require deeper investigation.
Another question to be addressed is whether the underlying mechanisms by which chronic RT improves muscle glucose regulation are the same as those utilized by chronic ET. It is necessary to determine which pathways are recruited by each type of exercise training, once again due to the possibility that one training method may impart greater benefit to certain individuals than to others as a result of their genetic makeup, physiology, and current medication use. Differences in sex, age and ethnicity likely contribute to the distinct outcomes of several studies looking at the metabolic effects of endurance and resistance training, even when the exercise protocol itself remains the same. While some studies report beneficial effects of RT on diverse metabolic parameters [19, 88, 89], others do not [90, 91]. It is therefore critical to report the training load, duration and relative intensity when comparing different groups of individuals. Addressing why discrepancies exist in outcomes from studies where individuals trained at similar intensities and duration may hold the key to effectively incorporate exercise training in the treatment of metabolic disease on a personalized level.
RT is a promising strategy to promote overall metabolic health in individuals with T2D via improvements in muscle mitochondrial performance and increases in muscle mass that may positively impact insulin responsiveness and glucose control. Multifactorial events contribute to the beneficial adaptations elicited by chronic RT (Fig. 1). A consistent regimen of RT at moderate intensity elicits the most beneficial metabolic responses—in terms of HbA1c and insulin sensitivity—in individuals with T2D. Current exercise recommendations should ideally be a synthesis of gains in muscle mass (higher intensity RT) and mitochondrial oxidative capacity (lower intensity RT or ET), as well as aim to reduce circulating pro-inflammatory cytokines (targeting WAT), in order to fully exploit the salutary effects of training on overall metabolic health. This can most efficiently be achieved by utilizing combined (RT and ET) training regimes. Another important consideration for current diabetes treatment guidelines is the use of metformin in combination with exercise training as the question of synergism vs. antagonism remains unanswered (Fig. 2). Combinations of lifestyle interventions (e.g., such as medication use, caloric restriction and weight loss) may offer additional benefits and additive effects to a RT regime.
We want to thank Dr. Varman Samuel for insightful discussions.
This work was supported by the Austrian Science Fund (FWF), project no. J 3267 and American Diabetes Association Junior Faculty Award (#7-13-JF-53).
Availability of data and materials
Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.
DHP conceptualized and wrote the manuscript and designed the figures. RLSG and AKM wrote the manuscript and gave input to the figures. BS, AKM and LMS wrote and proofread the manuscript and gave input to the figures. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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- Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract. 2014;103:137–49.View ArticlePubMedGoogle Scholar
- Long AN, Dagogo-Jack S. Comorbidities of diabetes and hypertension: mechanisms and approach to target organ protection. J Clin Hypertens (Greenwich). 2011;13:244–51.View ArticleGoogle Scholar
- Hordern MD, Dunstan DW, Prins JB, Baker MK, Singh MA, Coombes JS. Exercise prescription for patients with type 2 diabetes and pre-diabetes: a position statement from Exercise and Sport Science Australia. J Sci Med Sport. 2012;15:25–31.View ArticlePubMedGoogle Scholar
- Colberg SR, Sigal RJ, Fernhall B, Regensteiner JG, Blissmer BJ, Rubin RR, Chasan-Taber L, Albright AL, Braun B, American College of Sports M, American Diabetes A. Exercise and type 2 diabetes: the American College of Sports Medicine and the American Diabetes Association: joint position statement executive summary. Diabetes Care. 2010;33:2692–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Madden KM. Evidence for the benefit of exercise therapy in patients with type 2 diabetes. Diabetes Metab Syndr Obes. 2013;6:233–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Colberg SR, Sigal RJ, Yardley JE, Riddell MC, Dunstan DW, Dempsey PC, Horton ES, Castorino K, Tate DF. Physical activity/exercise and diabetes: a position statement of the American diabetes association. Diabetes Care. 2016;39:2065–79.View ArticlePubMedGoogle Scholar
- Schwingshackl L, Missbach B, Dias S, Konig J, Hoffmann G. Impact of different training modalities on glycaemic control and blood lipids in patients with type 2 diabetes: a systematic review and network meta-analysis. Diabetologia. 2014;57:1789–97.View ArticlePubMedGoogle Scholar
- Mann S, Beedie C, Balducci S, Zanuso S, Allgrove J, Bertiato F, Jimenez A. Changes in insulin sensitivity in response to different modalities of exercise: a review of the evidence. Diabetes Metab Res Rev. 2014;30:257–68.View ArticlePubMedGoogle Scholar
- Richter EA, Turcotte L, Hespel P, Kiens B. Metabolic responses to exercise. Effects of endurance training and implications for diabetes. Diabetes Care. 1992;15:1767–76.View ArticlePubMedGoogle Scholar
- Devries MC, Hamadeh MJ, McCready C, Sischek S, Tarnopolsky MA. Twelve weeks of endurance training increases mitochondrial density and percent IMCL touching mitochondria and alters IMCL storage distribution. FASEB Journal. 2008;22:753.718.Google Scholar
- Bacchi E, Negri C, Targher G, Faccioli N, Lanza M, Zoppini G, Zanolin E, Schena F, Bonora E, Moghetti P. Both resistance training and aerobic training reduce hepatic fat content in type 2 diabetic subjects with nonalcoholic fatty liver disease (the RAED2 randomized trial). Hepatology. 2013;58(4):1287–95.View ArticlePubMedGoogle Scholar
- Church TS, Blair SN, Cocreham S, Johannsen N, Johnson W, Kramer K, Mikus CR, Myers V, Nauta M, Rodarte RQ, et al. Effects of aerobic and resistance training on hemoglobin A1c levels in patients with type 2 diabetes: a randomized controlled trial. JAMA. 2010;304:2253–62.View ArticlePubMedPubMed CentralGoogle Scholar
- Sparks LM, Johannsen NM, Church TS, Earnest CP, Moonen-Kornips E, Moro C, Hesselink MK, Smith SR, Schrauwen P. Nine months of combined training improves ex vivo skeletal muscle metabolism in individuals with type 2 diabetes. J Clin Endocrinol Metab. 2013;98:1694–702.View ArticlePubMedPubMed CentralGoogle Scholar
- DeFronzo RA, Tripathy D. Skeletal muscle insulin resistance is the primary defect in type 2 diabetes. Diabetes Care. 2009;32 Suppl 2:S157–63.View ArticlePubMedPubMed CentralGoogle Scholar
- DeFronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP. The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization. Diabetes. 1981;30:1000–7.View ArticlePubMedGoogle Scholar
- Kelley DE, He J, Menshikova EV, Ritov VB. Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes. Diabetes. 2002;51:2944–50.View ArticlePubMedGoogle Scholar
- Romero-Arenas S, Martinez-Pascual M, Alcaraz PE. Impact of resistance circuit training on neuromuscular, cardiorespiratory and body composition adaptations in the elderly. Aging Dis. 2013;4:256–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Goodman CA. The role of mTORC1 in regulating protein synthesis and skeletal muscle mass in response to various mechanical stimuli. Rev Physiol Biochem Pharmacol. 2014;166:43–95.PubMedGoogle Scholar
- Holten MK, Zacho M, Gaster M, Juel C, Wojtaszewski JF, Dela F. Strength training increases insulin-mediated glucose uptake, GLUT4 content, and insulin signaling in skeletal muscle in patients with type 2 diabetes. Diabetes. 2004;53:294–305.View ArticlePubMedGoogle Scholar
- McKinsey TA, Zhang CL, Lu J, Olson EN: Signal-dependent nuclear export of a histone deacetylase regulates muscle differentiation. Nature. 2000;408:106–111.Google Scholar
- Hortobagyi T, Dempsey L, Fraser D, Zheng D, Hamilton G, Lambert J, Dohm L. Changes in muscle strength, muscle fibre size and myofibrillar gene expression after immobilization and retraining in humans. J Physiol. 2000;524(Pt 1):293–304.View ArticlePubMedPubMed CentralGoogle Scholar
- Harmer AR, Elkins MR. Amount and frequency of exercise affect glycaemic control more than exercise mode or intensity. Br J Sports Med. 2015;49:1012–4.View ArticlePubMedGoogle Scholar
- Yki-Jarvinen H, Koivisto VA. Effects of body composition on insulin sensitivity. Diabetes. 1983;32:965–9.View ArticlePubMedGoogle Scholar
- Ishii T, Yamakita T, Sato T, Tanaka S, Fujii S. Resistance training improves insulin sensitivity in NIDDM subjects without altering maximal oxygen uptake. Diabetes Care. 1998;21:1353–5.View ArticlePubMedGoogle Scholar
- Castorena CM, Arias EB, Sharma N, Bogan JS, Cartee GD. Fiber type effects on contraction-stimulated glucose uptake and GLUT4 abundance in single fibers from rat skeletal muscle. Am J Physiol Endocrinol Metab. 2015;308:E223–30.View ArticlePubMedGoogle Scholar
- Mackrell JG, Cartee GD. A novel method to measure glucose uptake and myosin heavy chain isoform expression of single fibers from rat skeletal muscle. Diabetes. 2012;61:995–1003.View ArticlePubMedPubMed CentralGoogle Scholar
- Holloszy JO, Hansen PA. Regulation of glucose transport into skeletal muscle. Rev Physiol Biochem Pharmacol. 1996;128:99–193.PubMedGoogle Scholar
- Vollestad NK, Blom PC, Gronnerod O. Resynthesis of glycogen in different muscle fibre types after prolonged exhaustive exercise in man. Acta Physiol Scand. 1989;137:15–21.View ArticlePubMedGoogle Scholar
- Schofield KL, Rehrer NJ, Perry TL, Ross A, Andersen JL, Osborne H. Insulin and fiber type in the offspring of T2DM subjects with resistance training and detraining. Med Sci Sports Exerc. 2012;44:2331–9.View ArticlePubMedGoogle Scholar
- Geisler S, Brinkmann C, Schiffer T, Kreutz T, Bloch W, Brixius K. The influence of resistance training on patients with metabolic syndrome--significance of changes in muscle fiber size and muscle fiber distribution. J Strength Cond Res. 2011;25:2598–604.View ArticlePubMedGoogle Scholar
- Adams GR, Hather BM, Baldwin KM, Dudley GA. Skeletal muscle myosin heavy chain composition and resistance training. J Appl Physiol (1985). 1993;74:911–5.Google Scholar
- Lowell BB, Shulman GI. Mitochondrial dysfunction and type 2 diabetes. Science. 2005;307:384–7.View ArticlePubMedGoogle Scholar
- Sparks LM, Xie H, Koza RA, Mynatt R, Hulver MW, Bray GA, Smith SR. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle. Diabetes. 2005;54:1926–33.View ArticlePubMedGoogle Scholar
- Boushel R, Gnaiger E, Schjerling P, Skovbro M, Kraunsoe R, Dela F. Patients with type 2 diabetes have normal mitochondrial function in skeletal muscle. Diabetologia. 2007;50:790–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Hesselink MK, Schrauwen-Hinderling V, Schrauwen P. Skeletal muscle mitochondria as a target to prevent or treat type 2 diabetes mellitus. Nat Rev Endocrinol. 2016;12:633–45.View ArticlePubMedGoogle Scholar
- Akimoto T, Pohnert SC, Li P, Zhang M, Gumbs C, Rosenberg PB, Williams RS, Yan Z. Exercise stimulates Pgc-1alpha transcription in skeletal muscle through activation of the p38 MAPK pathway. J Biol Chem. 2005;280:19587–93.View ArticlePubMedGoogle Scholar
- Frank P, Andersson E, Ponten M, Ekblom B, Ekblom M, Sahlin K. Strength training improves muscle aerobic capacity and glucose tolerance in elderly. Scand J Med Sci Sports. 2016;26:764–73.Google Scholar
- Irving BA, Lanza IR, Henderson GC, Rao RR, Spiegelman BM, Nair KS. Combined training enhances skeletal muscle mitochondrial oxidative capacity independent of age. J Clin Endocrinol Metab. 2015;100:1654–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E. Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol. 2011;301:R1078–87.View ArticlePubMedGoogle Scholar
- Tang JE, Hartman JW, Phillips SM. Increased muscle oxidative potential following resistance training induced fibre hypertrophy in young men. Appl Physiol Nutr Metab. 2006;31:495–501.View ArticlePubMedGoogle Scholar
- Colberg SR, Albright AL, Blissmer BJ, Braun B, Chasan-Taber L, Fernhall B, Regensteiner JG, Rubin RR, Sigal RJ, American College of Sports M, American Diabetes A. Exercise and type 2 diabetes: American College of Sports Medicine and the American Diabetes Association: joint position statement. Exercise and type 2 diabetes. Med Sci Sports Exerc. 2010;42:2282–303.View ArticlePubMedGoogle Scholar
- Chung N, Kreutz T, Schiffer T, Opitz D, Hermann R, Gehlert S, Bloch W, Brixius K, Brinkmann C. Training-induced alterations of skeletal muscle mitochondrial biogenesis proteins in non-insulin-dependent type 2 diabetic men. Can J Physiol Pharmacol. 2012;90:1634–41.View ArticlePubMedGoogle Scholar
- Hawley JA. Molecular responses to strength and endurance training: are they incompatible? Appl Physiol Nutr Metab. 2009;34:355–61.View ArticlePubMedGoogle Scholar
- Wang L, Mascher H, Psilander N, Blomstrand E, Sahlin K. Resistance exercise enhances the molecular signaling of mitochondrial biogenesis induced by endurance exercise in human skeletal muscle. J Appl Physiol (1985). 2011;111:1335–44.View ArticleGoogle Scholar
- Lantier L, Fentz J, Mounier R, Leclerc J, Treebak JT, Pehmoller C, Sanz N, Sakakibara I, Saint-Amand E, Rimbaud S, et al. AMPK controls exercise endurance, mitochondrial oxidative capacity, and skeletal muscle integrity. FASEB J. 2014;28:3211–24.View ArticlePubMedGoogle Scholar
- McGee SL, Hargreaves M. AMPK-mediated regulation of transcription in skeletal muscle. Clin Sci (Lond). 2010;118:507–18.View ArticleGoogle Scholar
- Gross DN vHA, Birnbaum NJ. The role of FoxO in the regulation of metabolism. Oncogene. 2008;27:2320–36.View ArticlePubMedGoogle Scholar
- Tarnopolsky MA. Mitochondrial DNA shifting in older adults following resistance exercise training. Appl Physiol Nutr Metab. 2009;34:348–54.View ArticlePubMedGoogle Scholar
- Parise G, Brose AN, Tarnopolsky MA. Resistance exercise training decreases oxidative damage to DNA and increases cytochrome oxidase activity in older adults. Exp Gerontol. 2005;40:173–80.View ArticlePubMedGoogle Scholar
- McInnes J. Mitochondrial-associated metabolic disorders: foundations, pathologies and recent progress. Nutr Metab (Lond). 2013;10:63.View ArticleGoogle Scholar
- Rodell A, Rasmussen LJ, Bergersen LH, Singh KK, Gjedde A. Natural selection of mitochondria during somatic lifetime promotes healthy aging. Front Neuroenergetics. 2013;5:7.View ArticlePubMedPubMed CentralGoogle Scholar
- Lefort N, Glancy B, Bowen B, Willis WT, Bailowitz Z, De Filippis EA, Brophy C, Meyer C, Hojlund K, Yi Z, Mandarino LJ. Increased reactive oxygen species production and lower abundance of complex I subunits and carnitine palmitoyltransferase 1B protein despite normal mitochondrial respiration in insulin-resistant human skeletal muscle. Diabetes. 2010;59:2444–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Houstis N, Rosen ED, Lander ES. Reactive oxygen species have a causal role in multiple forms of insulin resistance. Nature. 2006;440:944–8.View ArticlePubMedGoogle Scholar
- Goncalves RL, Bunik VI, Brand MD. Production of superoxide/hydrogen peroxide by the mitochondrial 2-oxoadipate dehydrogenase complex. Free Radic Biol Med. 2016;91:247–255.Google Scholar
- Goncalves RL, Quinlan CL, Perevoshchikova IV, Hey-Mogensen M, Brand MD. Sites of superoxide and hydrogen peroxide production by muscle mitochondria assessed ex vivo under conditions mimicking rest and exercise. J Biol Chem. 2015;290:209–27.View ArticlePubMedGoogle Scholar
- McBride JM, Kraemer WJ, Triplett-McBride T, Sebastianelli W. Effect of resistance exercise on free radical production. Med Sci Sports Exerc. 1998;30:67–72.View ArticlePubMedGoogle Scholar
- Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000;32:1576–81.View ArticlePubMedGoogle Scholar
- Petersen AC, McKenna MJ, Medved I, Murphy KT, Brown MJ, Della Gatta P, Cameron-Smith D. Infusion with the antioxidant N-acetylcysteine attenuates early adaptive responses to exercise in human skeletal muscle. Acta Physiol (Oxf). 2012;204:382–92.View ArticleGoogle Scholar
- Whitham M, Chan MH, Pal M, Matthews VB, Prelovsek O, Lunke S, El-Osta A, Broenneke H, Alber J, Bruning JC, et al. Contraction-induced interleukin-6 gene transcription in skeletal muscle is regulated by c-Jun terminal kinase/activator protein-1. J Biol Chem. 2012;287:10771–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Rietjens SJ, Beelen M, Koopman R, LJ VANL, Bast A, Haenen GR. A single session of resistance exercise induces oxidative damage in untrained men. Med Sci Sports Exerc. 2007;39:2145–51.View ArticlePubMedGoogle Scholar
- Vincent HK, Bourguignon C, Vincent KR. Resistance training lowers exercise-induced oxidative stress and homocysteine levels in overweight and obese older adults. Obesity (Silver Spring). 2006;14:1921–30.View ArticleGoogle Scholar
- Vinetti G, Mozzini C, Desenzani P, Boni E, Bulla L, Lorenzetti I, Romano C, Pasini A, Cominacini L, Assanelli D. Supervised exercise training reduces oxidative stress and cardiometabolic risk in adults with type 2 diabetes: a randomized controlled trial. Sci Rep. 2015;5:9238.View ArticlePubMedPubMed CentralGoogle Scholar
- Leeuwenburgh C, Hansen PA, Holloszy JO, Heinecke JW. Hydroxyl radical generation during exercise increases mitochondrial protein oxidation and levels of urinary dityrosine. Free Radic Biol Med. 1999;27:186–92.View ArticlePubMedGoogle Scholar
- Hey-Mogensen M, Hojlund K, Vind BF, Wang L, Dela F, Beck-Nielsen H, Fernstrom M, Sahlin K. Effect of physical training on mitochondrial respiration and reactive oxygen species release in skeletal muscle in patients with obesity and type 2 diabetes. Diabetologia. 2010;53:1976–85.View ArticlePubMedGoogle Scholar
- Lee S, Tak E, Lee J, Rashid MA, Murphy MP, Ha J, Kim SS. Mitochondrial H2O2 generated from electron transport chain complex I stimulates muscle differentiation. Cell Res. 2011;21:817–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Sano M, Fukuda K. Activation of mitochondrial biogenesis by hormesis. Circ Res. 2008;103:1191–3.View ArticlePubMedGoogle Scholar
- Brinkmann C, Chung N, Schmidt U, Kreutz T, Lenzen E, Schiffer T, Geisler S, Graf C, Montiel-Garcia G, Renner R, et al. Training alters the skeletal muscle antioxidative capacity in non-insulin-dependent type 2 diabetic men. Scand J Med Sci Sports. 2012;22:462–70.View ArticlePubMedGoogle Scholar
- Merry TL, Ristow M. Do antioxidant supplements interfere with skeletal muscle adaptation to exercise training? J Physiol. 2016;594:5135–47.View ArticlePubMedGoogle Scholar
- Braun B, Eze P, Stephens BR, Hagobian TA, Sharoff CG, Chipkin SR, Goldstein B. Impact of metformin on peak aerobic capacity. Appl Physiol Nutr Metab. 2008;33:61–7.View ArticlePubMedGoogle Scholar
- Malin SK, Nightingale J, Choi SE, Chipkin SR, Braun B. Metformin modifies the exercise training effects on risk factors for cardiovascular disease in impaired glucose tolerant adults. Obesity (Silver Spring). 2013;21:93–100.View ArticleGoogle Scholar
- Sharoff CG, Hagobian TA, Malin SK, Chipkin SR, Yu H, Hirshman MF, Goodyear LJ, Braun B. Combining short-term metformin treatment and one bout of exercise does not increase insulin action in insulin-resistant individuals. Am J Physiol Endocrinol Metab. 2010;298:E815–23.View ArticlePubMedPubMed CentralGoogle Scholar
- Malin SK, Gerber R, Chipkin SR, Braun B. Independent and combined effects of exercise training and metformin on insulin sensitivity in individuals with prediabetes. Diabetes Care. 2012;35:131–6.View ArticlePubMedGoogle Scholar
- Malin SK, Braun B. Impact of metformin on exercise-induced metabolic adaptations to lower type 2 diabetes risk. Exerc Sport Sci Rev. 2016;44:4–11.View ArticlePubMedGoogle Scholar
- Hawley SA, Gadalla AE, Olsen GS, Hardie DG. The antidiabetic drug metformin activates the AMP-activated protein kinase cascade via an adenine nucleotide-independent mechanism. Diabetes. 2002;51:2420–5.View ArticlePubMedGoogle Scholar
- Richter EA, Ruderman NB. AMPK and the biochemistry of exercise: implications for human health and disease. Biochem J. 2009;418:261–75.View ArticlePubMedPubMed CentralGoogle Scholar
- Boule NG, Kenny GP, Larose J, Khandwala F, Kuzik N, Sigal RJ. Does metformin modify the effect on glycaemic control of aerobic exercise, resistance exercise or both? Diabetologia. 2013;56:2378–82.View ArticlePubMedGoogle Scholar
- Malin SK, Stephens BR, Sharoff CG, Hagobian TA, Chipkin SR, Braun B. Metformin’s effect on exercise and postexercise substrate oxidation. Int J Sport Nutr Exerc Metab. 2010;20:63–71.View ArticlePubMedGoogle Scholar
- Madiraju AK, Erion DM, Rahimi Y, Zhang XM, Braddock DT, Albright RA, Prigaro BJ, Wood JL, Bhanot S, MacDonald MJ, et al. Metformin suppresses gluconeogenesis by inhibiting mitochondrial glycerophosphate dehydrogenase. Nature. 2014;510:542–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Bridges HR, Jones AJ, Pollak MN, Hirst J. Effects of metformin and other biguanides on oxidative phosphorylation in mitochondria. Biochem J. 2014;462:475–87.View ArticlePubMedPubMed CentralGoogle Scholar
- Pereira SS, Alvarez-Leite JI. Low-grade inflammation, obesity, and diabetes. Curr Obes Rep. 2014;3:422–31.View ArticlePubMedGoogle Scholar
- Kwon H, Pessin JE. Adipokines mediate inflammation and insulin resistance. Front Endocrinol (Lausanne). 2013;4:71.Google Scholar
- El-Kader SMA. Aerobic versus resistance exercise training in modulation of insulin resistance, adipocytokines and inflammatory cytokine levels in obese type 2 diabetic patients. Journal of Advanced Research. 2011;2:179–83.View ArticleGoogle Scholar
- Shultz SP, Dahiya R, Leong GM, Rowlands DS, Hills AP, Byrne NM. Muscular strength, aerobic capacity, and adipocytokines in obese youth after resistance training: A pilot study. Australas Med J. 2015;8:113–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Jorge ML, de Oliveira VN, Resende NM, Paraiso LF, Calixto A, Diniz AL, Resende ES, Ropelle ER, Carvalheira JB, Espindola FS, et al. The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines, and muscle insulin signaling in patients with type 2 diabetes mellitus. Metabolism. 2011;60:1244–52.View ArticlePubMedGoogle Scholar
- Gillette RCB CA, Melby CL. Postexercise energy expenditure in response to acute aerobic or resistive exercise. Int J Sport Nutr Exerc Metab. 1994;4:347–60.View ArticleGoogle Scholar
- Elliot DL, Goldberg L, Kuehl KS. Effect of resistance training on excess post-exercise oxygen consumption. J Appl Sport Sci Res. 1992;6:77–81.Google Scholar
- Ormsbee MJ, Thyfault JP, Johnson EA, Kraus RM, Choi MD, Hickner RC. Fat metabolism and acute resistance exercise in trained men. J Appl Physiol (1985). 2007;102:1767–72.View ArticleGoogle Scholar
- Castaneda C, Layne JE, Munoz-Orians L, Gordon PL, Walsmith J, Foldvari M, Roubenoff R, Tucker KL, Nelson ME. A randomized controlled trial of resistance exercise training to improve glycemic control in older adults with type 2 diabetes. Diabetes Care. 2002;25:2335–41.View ArticlePubMedGoogle Scholar
- Cauza E, Hanusch-Enserer U, Strasser B, Ludvik B, Metz-Schimmerl S, Pacini G, Wagner O, Georg P, Prager R, Kostner K, et al. The relative benefits of endurance and strength training on the metabolic factors and muscle function of people with type 2 diabetes mellitus. Arch Phys Med Rehabil. 2005;86:1527–33.View ArticlePubMedGoogle Scholar
- Kwon HR, Han KA, Ku YH, Ahn HJ, Koo BK, Kim HC, Min KW. The effects of resistance training on muscle and body fat mass and muscle strength in type 2 diabetic women. Korean Diabetes J. 2010;34:101–10.View ArticlePubMedPubMed CentralGoogle Scholar
- Bateman LA, Slentz CA, Willis LH, Shields AT, Piner LW, Bales CW, Houmard JA, Kraus WE. Comparison of aerobic versus resistance exercise training effects on metabolic syndrome (from the Studies of a Targeted Risk Reduction Intervention Through Defined Exercise - STRRIDE-AT/RT). Am J Cardiol. 2011;108:838–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metab. 2013;17:162–84.View ArticlePubMedGoogle Scholar